Protein Info for BT2851 in Bacteroides thetaiotaomicron VPI-5482

Annotation: alpha-galactosidase (melibiase) (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 721 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details PF16875: Glyco_hydro_36N" amino acids 64 to 269 (206 residues), 156.9 bits, see alignment E=1e-49 PF02065: Melibiase" amino acids 275 to 615 (341 residues), 349.4 bits, see alignment E=3e-108 PF16874: Glyco_hydro_36C" amino acids 630 to 716 (87 residues), 67.1 bits, see alignment E=2.4e-22

Best Hits

KEGG orthology group: K07407, alpha-galactosidase [EC: 3.2.1.22] (inferred from 100% identity to bth:BT_2851)

Predicted SEED Role

"Alpha-galactosidase (EC 3.2.1.22)" in subsystem Fructooligosaccharides(FOS) and Raffinose Utilization or Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Melibiose Utilization (EC 3.2.1.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.22

Use Curated BLAST to search for 3.2.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A3V3 at UniProt or InterPro

Protein Sequence (721 amino acids)

>BT2851 alpha-galactosidase (melibiase) (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MYIMKVRILFLICFLSITCLLNAADKPVIQIHTDNSSLIFRVADNGRLYQSYLGKKLNHE
ADISHLPQGTEAYITHGMEDYFEPAIHILHNDGNPSLLLKYVSHETKAVSQGVNETIITL
GDDKYPVTVKLHYVTYPAEDIIKTYTEISHKEKKPVVLHQYASSMLHLNRAKYYLTEFSG
DWAHEANISDQPLEFGKKVLDTKLGARANMFTPPFFQLSLDQLATENCGEVLVGTLGWTG
NFRFTFEVDNKNELRIISGINPYASEYYLPAGVVFRTPDFYFTYSANGKGKASRNFHDWA
RRYQLKDGDETRMTLLNNWEATYFDFNEEKLIGLIGDAAGLGVDMFLLDDGWFANKYPRS
SDHQGLGDWDETADKLPNGIGRLVEEATKKGIKFGLWIEPEMVNPKSELYEKHKDWVIHL
PNRDEYYFRNQLVLDLSNPKVQDFVFGVVDDLMTKYPGIAFFKWDCNSPITNIYSSYLKE
KQSHLYIDYVRGLYNVLERIKTKYPDLPMMLCSGGSGRIDYETLRYFTEFWPSDNTDPIE
RLFIQWGYSQLFPAKTLCAHVTTWNHDASIKFRTDVAMMGKLGFDIKLSDLNDNEKKFCR
DAVTNYNHLKPVVLEGDMYRLVSPYSGNHTSTQYVGKDKKGAVVFAFDIYSRYGEKLLPV
RLQGLDAGRQYRVKEINLMPGTNSSLQGNGELFSGEYLMTVGLNIFTGQRLNSRVIEVVA
E