Protein Info for BT2563 in Bacteroides thetaiotaomicron VPI-5482

Annotation: polyribonucleotide nucleotidyltransferase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 708 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 13 to 700 (688 residues), 970.7 bits, see alignment E=2.3e-296 PF01138: RNase_PH" amino acids 14 to 145 (132 residues), 100.6 bits, see alignment E=2.3e-32 amino acids 327 to 457 (131 residues), 85.4 bits, see alignment E=1.2e-27 PF03725: RNase_PH_C" amino acids 148 to 212 (65 residues), 58.4 bits, see alignment E=1.4e-19 PF03726: PNPase" amino acids 244 to 324 (81 residues), 47.5 bits, see alignment E=5.1e-16 PF00013: KH_1" amino acids 560 to 620 (61 residues), 51.3 bits, see alignment 2e-17 PF00575: S1" amino acids 626 to 699 (74 residues), 61.3 bits, see alignment E=2.3e-20

Best Hits

Swiss-Prot: 100% identical to PNP_BACTN: Polyribonucleotide nucleotidyltransferase (pnp) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 100% identity to bth:BT_2563)

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A4N6 at UniProt or InterPro

Protein Sequence (708 amino acids)

>BT2563 polyribonucleotide nucleotidyltransferase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MINPIVKTIELPDGRTITLETGKLAKQADGSVMLRMGNTMLLATVCAAKDAVPGTDFMPL
QVEYKEKFAAFGRFPGGFTKREGRASDYEILTCRLVDRALRPLFPDNYHAEVYVNIILFS
ADGVDMPDALAGLAASAALAVSDIPFNGPISEVRVARIDGQFVINPTFDQLEKADMDLMV
AATYDNIMMVEGEMHEVSEAELLEAMKVAHEAIKVHCKAQMELTEEVGKTVKREYNHEVN
DEELRKAVREACYDKAYAVAASGNNNKHERFDAFDAIREEFKAQFSEEELEEKAPLIDRY
YHDVEKEAMRRSILDEGKRLDGRKTTEIRPIWCEVGPLPGPHGSAIFTRGETQSLTSVTL
GTKLDEKIIDNVLEHGKERFLLHYNFPPFSTGEAKAQRGVGRREIGHGHLAWRALKGQIP
ADYPYVVRVVSEILESNGSSSMATVCAGTLALMDAGVKIKKPVSGIAMGLIKNPGEEKYA
VLSDILGDEDHLGDMDFKVTGTRDGITATQMDIKVDGLSYEILERALNQAKEGRMHILDK
ITETIAEPRADLKDHAPRIETMTIPKEFIGAVIGPGGKIIQGMQEETGAVITIEETDGMG
RIEVSGTNKKCIDDAMRMIKAIVAVPEVGEVYKGKVRSIMPYGAFIEFLPGKDGLLHISE
IDWKRLETVEEAGIKEGDEIEVKLIDIDPKTGKFKLSRKVLMPRPEKK