Protein Info for BT2501 in Bacteroides thetaiotaomicron VPI-5482

Annotation: penicillin-binding protein (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: None (inferred from 100% identity to bth:BT_2501)

Predicted SEED Role

"Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)" in subsystem Peptidoglycan Biosynthesis or Type IV pilus (EC 2.4.1.129, EC 3.4.-.-)

MetaCyc Pathways

peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)
peptidoglycan maturation (meso-diaminopimelate containing) (4/12 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.129, 3.4.-.-

Use Curated BLAST to search for 2.4.1.129 or 3.4.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A4U6 at UniProt or InterPro

Protein Sequence (649 amino acids)

>BT2501 penicillin-binding protein (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MKKNSKKSILLLSIGGGLFICLISIYLSRNMLLQSITNKRTTHIEQTYGLQIHYQNLQMK
GCSEITLQGLSIVPNQRDTLLTLQSVNVRLNFWKLLKGNIEVRNVHMNGLAIAFIKRDSA
ANYDFLFSGHHPEATTEPVIETNYAHRINRILNLIYGFFPENGQLTQLNITERKDSNFVT
VNIPTFTIENNRFQSTIKIKEDTLTQQWKVAGELNRKVHTLQAELFATEQKKVSLPYINR
RFGAEVTFDTLYYSMTKENRTENQLQLDGTAKVNGLDVFHKALSPEVIHLDRGQLTYQMN
IGKQTLELDSTTTVLFNQIKFHPYLRAEKNENQWHFTAATDKSWFPADELFSSLPKGLFS
NLEGIKTSGELAYHFLLDIDFARLDSLKFESELKEKDFRIIEYGATSLSKMSEEFIYTAY
ENGIPVKTFPVGPSWEHFTPLDSISPLLRMSVMQSEDGAFFYHKGFLPDAMREALIYDLQ
VERFARGGSTITMQLVKNVFLNRNKNFARKLEEALIVWLIETERLTSKERMYEVYLNIAE
WGPLVYGIQEASAYYFGKRPSQLTTEESIFLASIIPKPKHFRSSFAENGRLKENMEGYYK
LIAGRLAKKGLISEIEADSIRPDIQVTGDALNSLVGETPESSSPTAEEQ