Protein Info for BT2403 in Bacteroides thetaiotaomicron VPI-5482

Updated annotation (from data): aspartate kinase / homoserine dehydrogenase (EC 2.7.2.4; EC 1.1.1.3)
Rationale: Important for fitness in most defined media. 37% identical to a bifunctional aspartokinase/homoserine dehydrogenase from Arabidopsis (O81852).
Original annotation: aspartokinase/homoserine dehydrogenase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 811 transmembrane" amino acids 461 to 477 (17 residues), see Phobius details amino acids 789 to 808 (20 residues), see Phobius details PF00696: AA_kinase" amino acids 2 to 278 (277 residues), 126.7 bits, see alignment E=3.1e-40 TIGR00657: aspartate kinase" amino acids 3 to 455 (453 residues), 369.9 bits, see alignment E=1.1e-114 PF13840: ACT_7" amino acids 310 to 371 (62 residues), 32.3 bits, see alignment 1.8e-11 amino acids 387 to 452 (66 residues), 31.5 bits, see alignment E=3.3e-11 PF03447: NAD_binding_3" amino acids 468 to 603 (136 residues), 48.4 bits, see alignment E=3.5e-16 PF00742: Homoserine_dh" amino acids 611 to 806 (196 residues), 163.8 bits, see alignment E=9.2e-52

Best Hits

KEGG orthology group: K12524, bifunctional aspartokinase / homoserine dehydrogenase 1 [EC: 1.1.1.3 2.7.2.4] (inferred from 100% identity to bth:BT_2403)

Predicted SEED Role

"Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3)" in subsystem Lysine Biosynthesis DAP Pathway or Threonine and Homoserine Biosynthesis or Methionine Biosynthesis (EC 1.1.1.3, EC 2.7.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.2.4

Use Curated BLAST to search for 1.1.1.3 or 2.7.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A541 at UniProt or InterPro

Protein Sequence (811 amino acids)

>BT2403 aspartate kinase / homoserine dehydrogenase (EC 2.7.2.4; EC 1.1.1.3) (Bacteroides thetaiotaomicron VPI-5482)
MKVMKFGGTSVGSVNSILSVKRIVESAGEPVIVVVSALGGITDKLINTSKMAAAGDSAYE
GEFREIVYRHVEMIKEVIPAGAGQVALQRQIGELLNELKDIFQGIYLIRDLSPKTSDTIV
SYGERLSSIIVAELIDEAKWFDSRTFIKTEKKHNKHTIDADLTNQLVKEAFHSIPKVSLV
PGFISSDKVSGDVTNLGRGGSDYTAAIIAAALDASSLEIWTDVDGFMTADPRVISTAYTI
SELSYVEATELCNFGAKVVYPPTIYPVCHKNIPIIIKNTFNPDGVGTVIKQETSNPQSKA
IKGISSINDTSLITVQGLGMVGVIGVNYRIFKALAKNGISVFLVSQASSENSTSIGVRNA
DADLACEVLNEEFAKEIEMGEISPILAERNLATVAIVGENMKHTPGIAGKLFGTLGRNGI
NVIACAQGASETNISFVVDSKSLRKSLNVIHDSFFLSEYQVLNLFICGIGTVGGSLVEQI
RCQQQKLMMENGLKLHVVGIIDAAKAMFSREGFDLANFREELQKKGKDSNLQTIRDEIVG
MNIFNSVFVDCTASPDIASLYKDLLQHNVSVVAANKIAASSAYENYRELKTIARQRGVKY
LFETNVGAGLPIINTINDLIHSGDKILKIEAVLSGTLNYIFNKISADIPFSRTIKMAQEE
RYSEPDPRIDLSGKDVIRKLVILAREAGYHIEQEDVEKNLFVPNDFFEGSLDDFWKRVPS
LDADFEARRQVLEKEHKHWRFVAKLEDGKASVGLQEVGANHPFYGLEGSNNIILLTTERY
KEYPMMIQGYGAGAGVTAAGVFADIMSIANV