Protein Info for BT2199 in Bacteroides thetaiotaomicron VPI-5482

Annotation: alpha-1,2-mannosidase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 758 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR01180: putative alpha-1,2-mannosidase" amino acids 7 to 742 (736 residues), 757.7 bits, see alignment E=6.1e-232 PF17678: Glyco_hydro_92N" amino acids 31 to 257 (227 residues), 196.1 bits, see alignment E=8.9e-62 PF07971: Glyco_hydro_92" amino acids 263 to 738 (476 residues), 549 bits, see alignment E=1.1e-168

Best Hits

KEGG orthology group: None (inferred from 100% identity to bth:BT_2199)

MetaCyc: 100% identical to alpha-1,2-mannosidase (Bacteroides thetaiotaomicron)
Alpha-mannosidase. [EC: 3.2.1.24]

Predicted SEED Role

"Alpha-1,2-mannosidase" in subsystem Mannose Metabolism

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.24

Use Curated BLAST to search for 3.2.1.24

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A5N9 at UniProt or InterPro

Protein Sequence (758 amino acids)

>BT2199 alpha-1,2-mannosidase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MVTRLFFLSICFPFLLTSCQQSKKTVEFVDYVNPLMGTESTFAFSHGNTYPAVAVPWGMN
FWSPQTGENGSGWMYTYTDSLMRGFRQTHQPSPWINDYGTFSIMPLAGELKMSHKERLVP
FSHQQEKATPYNYSVTFNNGLQTSLSATSRGAVFEVSFPEKEDQYVVVDAYNGGSSITIE
PEKRLVKGATRYNNGGVPDNFANYFMMEFSHPVIEYGTYNGDTLLHHQTDVAADYTCAYL
KFDVPAGEKLTIRTASSFISPEQAAINFNREVADADVQLISGKAREQWNNYLGRVEAEGG
TDEQLRTFYSCLYRTLLFPREFYEFDSQGNPVYYSPYDGNVHDGYMYTDNGFWDTFRAVH
PLFTLLYPEVSERVTQSIINAYNESGFMPEWASPGHRGCMIGNNSVSLLVDAWMKGIQTV
DAEKALEAMIHQTQARHAEIASVGRDGFEYYDKLGYVPYPEVPEATAKTLEYAYADWCIA
RFAESLGKQDIADQYYQKAPNYRNLYYPEHGFMWTKDAKGNWRDRFDATEWGGPFTEGSS
WHWTWSVFHDPEGLSELMGGHEPMIARLDSMFVAPNTYNYGTYGFVIHEIAEMVALNMGQ
YAHGNQPVQHAIYLYDYIGQPWKTQYHLRNVMDKLYNSGSKGYCGDEDNGQTSAWYVFSA
MGFYPVCPGMPEYAIGSPLFKKVTLHLPEGKNFVVSAADNAADRPYIRKALLNGQEFTRN
YLTHDELKQGGELNLSMDSVPNQQRGTQPADFPYSYSK