Protein Info for BT2186 in Bacteroides thetaiotaomicron VPI-5482

Updated annotation (from data): 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19)
Rationale: This protein is essential, but a close homolog (HMPREF1058_RS13970) is cofit with other chorismate synthesis genes. Furthermore, this gene (also known as BT_RS11065) complemented an aroA- mutant of E. coli (data of PMID:39375541)
Original annotation: 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00275: EPSP_synthase
  • amino acids 9 to 59 (51 residues), 26.9 bits, 1e-10
  • amino acids 63 to 399 (337 residues), 244.2 bits, E=1.1e-76
• TIGR01356: 3-phosphoshikimate 1-carboxyvinyltransferase
  • amino acids 59 to 400 (342 residues), 287.7 bits, E=7.1e-90

Best Hits

Swiss-Prot: 77% identical to AROA_BACFN: 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) from Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / JCM 11019 / NCTC 9343)

KEGG orthology group: K00800, 3-phosphoshikimate 1-carboxyvinyltransferase [EC: 2.5.1.19] (inferred from 100% identity to bth:BT_2186)

Predicted SEED Role

"5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) (EC 2.5.1.19)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• superpathway of L-phenylalanine biosynthesis (10/10 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• chorismate biosynthesis I (7/7 steps found)
• chorismate biosynthesis from 3-dehydroquinate (5/5 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• superpathway of chorismate metabolism (38/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.19

Sequence Analysis Tools

See Q8A5Q2 at UniProt or InterPro

Protein Sequence (410 amino acids)

>BT2186 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) (Bacteroides thetaiotaomicron VPI-5482)
MMLYKLISPSMVKATIQLPASKSISNRALIINALGKGIYPPENLSDCDDTQVMIKALTEG
KETIDIMAAGTAMRFLTAYLSATSGERIITGTARMQQRPIQILVNALRELGAEIEYTHNE
GYPPLRIKGAELKGNEITLKGNVSSQYISALLMIGPVLKDGLTLHLTGEIISRPYINLTL
QLMQDFGAKAAWTSPSSISVAPQPYQSVPFTVESDWSAASYWYQIAALSPEAEIELLGLF
RNSYQGDSRGAEVFSRLGITTEFTPKGVKIKKTGKTPERLEEDFVDIPDLAQTFVVTCAL
LNIPFRFTGLQSLKIKETDRIAALRTELKKLGYLIEEENDSVLMWNGERCEPEAVPVIAT
YEDHRMAMAFAPAVITFPKLLIADPQVVSKSYPGYWEDLKLAGFQVINEG