Protein Info for BT1973 in Bacteroides thetaiotaomicron VPI-5482
Annotation: NAD-specific glutamate dehydrogenase (NCBI ptt file)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 96% identical to DHE2_BACFR: NAD-specific glutamate dehydrogenase (gdhB) from Bacteroides fragilis (strain YCH46)
KEGG orthology group: K00262, glutamate dehydrogenase (NADP+) [EC: 1.4.1.4] (inferred from 100% identity to bth:BT_1973)MetaCyc: 56% identical to glutamate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Glutamate dehydrogenase (NADP(+)). [EC: 1.4.1.4]
Predicted SEED Role
"NAD-specific glutamate dehydrogenase (EC 1.4.1.2)" in subsystem Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.2)
MetaCyc Pathways
- L-glutamate biosynthesis III (1/1 steps found)
- L-glutamate degradation I (1/1 steps found)
- L-glutamate and L-glutamine biosynthesis (5/7 steps found)
- L-alanine degradation II (to D-lactate) (2/3 steps found)
- ethene biosynthesis IV (engineered) (1/3 steps found)
- methylaspartate cycle (11/19 steps found)
- L-glutamate degradation XI (reductive Stickland reaction) (2/7 steps found)
- 4-aminobutanoate degradation V (1/7 steps found)
- L-glutamate degradation V (via hydroxyglutarate) (2/10 steps found)
KEGG Metabolic Maps
- Arginine and proline metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.2, 1.4.1.4
Use Curated BLAST to search for 1.4.1.2 or 1.4.1.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8A6B2 at UniProt or InterPro
Protein Sequence (445 amino acids)
>BT1973 NAD-specific glutamate dehydrogenase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482) MNIQKIMSSLEAKHPGELEYLQAVKEVLLSIEDIYNQHPEFEKVKIIERLVEPDRIFTFR VTWVDDKGEVQTNLGYRVQFNNAIGPYKGGIRFHASVNLSILKFLGFEQTFKNALTTLPM GGGKGGSDFSPRGKSDAEIMRFCQAFMLELWRHLGPDMDVPAGDIGVGGREVGYMFGMYK KLTREFTGTFTGKGLEFGGSLIRPEATGFGGLYFVYQMLQAKNIDIKGKTVAISGFGNVA WGAATKATELGAKVITISGPDGYIYDPDGISGEKIDYMLELRASGNDIVAPYAEKYPGAT FVEGKRPWEVKADIALPCATQNELNGEDAQNLIKNNVLCIGEISNMGCTPEAIDLFIEKK IMYAPGKAVNAGGVATSGLEMSQNAMHLSWSAAEVDEKLHAIMHGIHAQCVKYGTEPDGY INYVKGANIAGFMKVAHAMMGQGVI