Protein Info for BT1626 in Bacteroides thetaiotaomicron VPI-5482

Annotation: beta-galactosidase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1022 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF02837: Glyco_hydro_2_N" amino acids 70 to 244 (175 residues), 135 bits, see alignment E=8.2e-43 PF22666: Glyco_hydro_2_N2" amino acids 140 to 215 (76 residues), 31.1 bits, see alignment 7.4e-11 PF00703: Glyco_hydro_2" amino acids 247 to 356 (110 residues), 54.1 bits, see alignment E=7.4e-18 PF02836: Glyco_hydro_2_C" amino acids 359 to 641 (283 residues), 307 bits, see alignment E=4.1e-95 PF16353: LacZ_4" amino acids 653 to 739 (87 residues), 67.3 bits, see alignment E=3.5e-22 PF02929: Bgal_small_N" amino acids 754 to 1018 (265 residues), 227.8 bits, see alignment E=5.4e-71

Best Hits

KEGG orthology group: K01190, beta-galactosidase [EC: 3.2.1.23] (inferred from 100% identity to bth:BT_1626)

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8A799 at UniProt or InterPro

Protein Sequence (1022 amino acids)

>BT1626 beta-galactosidase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MKLKKRTFLILMAALTATFASAQKQPLPEWQSQYAVGLNKLAPHTYVWPYADASDIGKPG
GYEQSPYYMSLNGKWKFNWVKNPDNRPKDFYQPSYYTGGWADINVPGNWERQGYGTAIYV
NETYEFDDKMFNFKKNPPLVPFAENEVGSYRRTFKVPADWKGRRVVLCCEGVISFYYVWV
NGKLLGYNQGSKTAAEWDITDVLSEGENVVALEVYRWSSGAYLECQDMWRLSGIERDVYL
YSTPKQYIADYKVSASLDKEKYKEGIFNLEVTVEGPSATASSIAYTLKDASGKAVLQDAI
NIKSRGLSNFIAFDEKKIAEVKAWNAEHPNLYTLVLELKDAQGKVTELTGCEVGFRTSEI
KDGRFCINGVPVLVKGTNRHEHSQLGRTVSKELMEQDIRLMKQHNINMVRNSHYPTHPYW
YQLCDRYGLYMIDEANIESHGMGYGPASLAKDSTWLTAHMDRTHRMYERSKNHPAIVIWS
QGNEAGNGINFERTYDWLKSVEKGRPVQYERAELNYNTDIYCRMYRSVDEIKAYVGKKDI
YRPFILCEYLHAMGNSCGGMKEYWEVFENEPMAQGGCIWDWVDQNFREIDKDGKWYWTYG
GDYGPEGIPSFGNFCGNGLVNAVREPHPHLLEVKKIYQNIKATLSDRKNLKVCIKNWYDF
SNLNEYILRWNVKGEDGTVLAEGTKEVDCEPHATVDVTLGAVKLPNTVREAYLNLSWSRK
EATPLVDTDWEVAYDQFVLAGNKNTTAYRPQKAGETAFVVDKNTGALSSLTLDGKELLAA
PITLSLFRPATDNDNRDRNGARLWRKAGLNNLTQKVVSLKEEKTSATVRAEILNGKGQKV
GMADFVYALDKNGALKVRTTFQPDTAIVKSMARLGLTFRMADAYNQVSYLGRGDHETYID
RNQSGRIGLYDTTVERMFHYYATPQSTANRTDVRWAKLTDQAGEGVFMESNRPFQFSIIP
FSDVLLEKAHHINELERDGMITIHLDAEQAGVGTATCGPGVLPQYLVPVKKQSFEFTLYP
VK