Protein Info for BT0623 in Bacteroides thetaiotaomicron VPI-5482

Annotation: putative UDP-glucose 4-epimerase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01179: UDP-glucose 4-epimerase GalE
  • amino acids 4 to 339 (336 residues), 399.9 bits, E=3.6e-124
• PF04321: RmlD_sub_bind
  • amino acids 4 to 167 (164 residues), 32 bits, E=2.2e-11
• PF02719: Polysacc_synt_2
  • amino acids 5 to 127 (123 residues), 32.7 bits, E=1.4e-11
• PF01370: Epimerase
  • amino acids 5 to 268 (264 residues), 171.9 bits, E=4.8e-54
• PF01073: 3Beta_HSD
  • amino acids 6 to 263 (258 residues), 55.5 bits, E=1.4e-18
• PF16363: GDP_Man_Dehyd
  • amino acids 6 to 329 (324 residues), 164.5 bits, E=1.4e-51

Best Hits

Swiss-Prot: 51% identical to UGE5_ARATH: UDP-glucose 4-epimerase 5 (UGE5) from Arabidopsis thaliana

KEGG orthology group: K01784, UDP-glucose 4-epimerase [EC: 5.1.3.2] (inferred from 100% identity to bth:BT_0623)

MetaCyc: 51% identical to UDP-glucose 4-epimerase (Bacillus subtilis subtilis 168)
UDP-N-acetylglucosamine 4-epimerase. [EC: 5.1.3.7]; UDP-glucose 4-epimerase. [EC: 5.1.3.7, 5.1.3.2]

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• colanic acid building blocks biosynthesis (10/11 steps found)
• D-galactose degradation I (Leloir pathway) (4/5 steps found)
• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• D-galactose detoxification (2/3 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• stachyose degradation (4/7 steps found)
• UDP-sugars interconversion (4/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (11/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2 or 5.1.3.7

Sequence Analysis Tools

See Q8AA42 at UniProt or InterPro

Protein Sequence (344 amino acids)

>BT0623 putative UDP-glucose 4-epimerase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MKERILVTGGTGYIGSHTVVELQNSGYEVIIIDNLSNSSADVVDNIEKVSGIRPAFEKLD
CLDFAGLDAVFAKYKGIKAIIHFAASKAVGESVEKPLLYYRNNLVSLINLLELMPKHGVE
GIVFSSSCTVYGQPDELPVTEKAPIKKAESPYGNTKQINEEIIRDTIASGAPINAILLRY
FNPIGAHPTALLGELPNGVPQNLIPYLTQTAMGIREKLSVFGDDYDTPDGSCIRDFINVV
DLAKAHVIAIRRILEQKQKEKVEVFNIGTGRGVSVLELINGFEKATGVKLNYQIVGRRAG
DIEKVWANPDYANQELGWKAVETLEDTLRSAWNWQLKLRERGIQ