Protein Info for BT0553 in Bacteroides thetaiotaomicron VPI-5482
Annotation: glutamate synthase, large subunit (NCBI ptt file)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Predicted SEED Role
"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)
MetaCyc Pathways
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamine degradation II (1/1 steps found)
- L-glutamate and L-glutamine biosynthesis (5/7 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
- L-citrulline biosynthesis (5/8 steps found)
- L-asparagine biosynthesis III (tRNA-dependent) (1/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (1/4 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.13
Use Curated BLAST to search for 1.4.1.13
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8AAB2 at UniProt or InterPro
Protein Sequence (1536 amino acids)
>BT0553 glutamate synthase, large subunit (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482) MKKQELFNNATGKFPYQRQPGQMGLYDAAYEHDACGVGMLVNIHGEKSHDIVESALKVLE NMRHRGAEGADNKTGDGAGIMLQIPHEFILLQGIPVPEKGRYGTGLLFLPKNEKDQAAIF SIIIEEIEKEGLTLMHLRNVPTCPEILGEAALANEPDIKQVFITGFTETETADRKLYLIR KRIENKVRLSAIPTRNDFYVVSLSTKSIIYKGMLSSLQLRNYYPDLTNSYFTSGLALVHS RFSTNTFPTWGLAQPFRLLAHNGEINTIRGNRGWMEARESVLSTPMLGDIKEIRPIIQPG MSDSASLDNVLEFLVMSGLSLPHAMAMLVPESFNEKNPISEDLKAFYEYHSILMEPWDGP AALLFSDGRFAGGMLDRNGLRPARYLITKNDTMVVASEVGVMDFEPGDIKEKGRLQPGKI LLIDTEKGEIYYDGELKKQLAEAKPYRTWLSTNRIELDELKSGRKVPHHVENYDRMLRTF GYSKEDIERLIMPMASAGAEPIHSMGNDTPLAVLSDKPQLLYNYFRQQFAQVTNPPIDPL REELVMSLTEYIGAVGMNILTPSESHCKMVRLNHPILSNTQLDILCNIRYKGFKTVKLPM LFEVSKGKAGLQESLNNLCKMAEESVTDGANYIVLTDRDVDATHAVIPSLLAVSAVHHHL ISVGKRVQTALIVESGEMREVMHAALLLGFGASALNPYMAFAILDKLVKEKDIQLDYATA EKNYIKSICKGLFKIMSKMGISTIRSYRGAKIFEAVGLSEELSKAYFGGLGSPIGGIRLE EVARDAIAFHDEGVEGMENGELKMENEAPHGNSQFSTFNFPLLKNNGLYAFRKDGEKHAW NPETISTLQLATRLGSYKKFKEFTHLVDNKEKPIFLRDFLGFRRNPISIEQVEPIENILR RFVTGAMSFGSISKEAHEAMAIAMNTIHGRSNTGEGGEDASRFHPLPDGTSMRSAIKQVA SGRFGVTAEYLVNADEIQIKIAQGAKPGEGGQLPGFKVNDVIAKTRHSIPGISLISPPPH HDIYSIEDLAQLIFDLKNVNPQAKISVKLVAESGVGTIAAGVAKAKADLIVISGAEGGTG ASPASSIRYAGISPELGLSETQQTLVLNGLRGQVVLQADGQLKTGRDIIIMALMGAEEYG FATSALIVLGCVMMRKCHQNTCPVGVATQNEELRKRFHGRSEYLINFFTFLAQEVREYLA EMGFTKMDDIIGRTDLIERKSDENDPNPKHALIDFTKLLARVDNSAAIRHVIDQDHGIST VKDVAIIDAAQEAIEHEKEVSLEYTIANTDRATGAMLSGVIAKKHGEKGLPEHTLNVKFK GSAGQSFGAFLVPGVNFKLEGEANDYLGKGLSGGRIAVLPPIRSNFEAEKNTIAGNTLLY GATSGEVYINGRVGERFAVRNSGAVAVVEGVGDHCCEYMTGGRVVVLGQTGRNFAAGMSG GVAYVWNKDGNFDYFCNMEMVELSLIEEAGYRKELHELIRQHYLYTGSKLARTMLDDWNH YVDQFIQIVPIEYKKVLQEEQMRKLQQKIADMQRDY