Protein Info for BT0547 in Bacteroides thetaiotaomicron VPI-5482

Annotation: aspartate aminotransferase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to DAPAT_BACTN: LL-diaminopimelate aminotransferase (dapL) from Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)

KEGG orthology group: K10206, LL-diaminopimelate aminotransferase [EC: 2.6.1.83] (inferred from 100% identity to bth:BT_0547)

Predicted SEED Role

"L,L-diaminopimelate aminotransferase (EC 2.6.1.83)" in subsystem Lysine Biosynthesis DAP Pathway (EC 2.6.1.83)

MetaCyc Pathways

L-lysine biosynthesis VI (7/7 steps found)
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (12/15 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.6.1.83

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8AAB8 at UniProt or InterPro

Protein Sequence (410 amino acids)

>BT0547 aspartate aminotransferase (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MALVNEHFLKLPGSYLFSDIAKKVNTFKITHPKQDIIRLGIGDVTQPLPKACIEAMHKAV
EELASKDTFRGYGPEQGYDFLIEAIIKNDFAPRGIHFSPSEIFVNDGAKSDTGNIGDILR
HDNSVGVTDPIYPVYIDSNVMCGRAGVLEEGTGKWSNVTYMPCTSENDFIPEIPDKRIDI
VYLCYPNNPTGTTLTKPELKKWVDYALANDTLILFDAAYEAYIQDADVPHSIYEIKGAKK
CAIEFRSFSKTAGFTGVRCGYTVVPKELTAATLEGDRIPLNKLWNRRQCTKFNGTSYITQ
RAAEAVYSTEGKAQIKETINYYMSNAKIMKEGLEATGLKVYGGVNAPYLWVKTPNGLSSW
RFFEQMLYEANVVGTPGVGFGPSGEGYIRLTAFGDHNDCMEAMRRIKNWL