Protein Info for BT0125 in Bacteroides thetaiotaomicron VPI-5482

Annotation: NADH:ubiquinone oxidoreductase subunit (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 635 PF01257: 2Fe-2S_thioredx" amino acids 44 to 119 (76 residues), 34.8 bits, see alignment E=6.4e-12 PF01512: Complex1_51K" amino acids 197 to 368 (172 residues), 160.6 bits, see alignment E=1.2e-50 PF10531: SLBB" amino acids 392 to 440 (49 residues), 28.9 bits, see alignment 3.7e-10 PF10589: NADH_4Fe-4S" amino acids 480 to 563 (84 residues), 111.7 bits, see alignment E=5.7e-36 PF00037: Fer4" amino acids 580 to 603 (24 residues), 32.2 bits, see alignment (E = 3e-11) amino acids 611 to 632 (22 residues), 25.7 bits, see alignment (E = 3.5e-09) PF13237: Fer4_10" amino acids 581 to 627 (47 residues), 33.2 bits, see alignment 1.8e-11 PF12838: Fer4_7" amino acids 587 to 631 (45 residues), 28.9 bits, see alignment 5.8e-10 PF13187: Fer4_9" amino acids 587 to 631 (45 residues), 27.8 bits, see alignment 9.7e-10

Best Hits

KEGG orthology group: K00335, NADH dehydrogenase I subunit F [EC: 1.6.5.3] (inferred from 100% identity to bth:BT_0125)

MetaCyc: 63% identical to NADH-dependent hydrogenase beta subunit (Caldanaerobacter subterraneus tengcongensis)
Hydrogen dehydrogenase. [EC: 1.12.1.2]

Predicted SEED Role

"NADP-reducing [Fe]-hydrogenase, cytoplasmic, beta subunit (EC 1.12.1.4)" (EC 1.12.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.12.1.4, 1.6.5.3

Use Curated BLAST to search for 1.12.1.2 or 1.12.1.4 or 1.6.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8ABI5 at UniProt or InterPro

Protein Sequence (635 amino acids)

>BT0125 NADH:ubiquinone oxidoreductase subunit (NCBI ptt file) (Bacteroides thetaiotaomicron VPI-5482)
MKILSIHDLQIIRKRAEHHLSLREESNEKVSEKCCGLASGTTHLQILICGGTGCKASSSQ
GITENLQKAIERNGITDKVDVITVGCFGFCEKGPIVKIIPDNTFYTQVTPEDAEEIISEH
IIGGRRIERLLYIDPKTEQTVSDSKHMDFYRKQLRIALRNCGFIDSENIEEYIAREGYFA
LADCLLNKQPADVIDIIKRSGLRGRGGGGFPTGLKWEFASKQVSNVKYVVCNADEGDPGA
FMDRSIMEGDPHSIVEAMCICGYSIGSSKGLVYIRAEYPLAINRLKKAIEQAREYGLLGD
HILGTDFSFDIEIRYGAGAFVCGEETALIHSMEGKRGEPTLKPPFPAESGYLGKPTNVNN
VETLANIPIILTKGAEWFASIGTERSKGTKVFALAGKINNVGLIEVPMGTTLREVIYEIG
GGIKGGKKFKAVQTGGPSGGCLTEKHLDTPIDFDNLLAAGSMMGSGGMIVMDEDDCMVSV
ARFYLDFTVEESCGKCTPCRIGNKRLLELLNKITEGRGTEKDLDTLATLGRVIKDTALCG
LGQTSPNPVLSTLDNFRDEYLEHVRDKTCRAKQCKSLLTYTINPELCIGCHLCAKNCPAD
AISGLVRKPHVIHPEKCIKCGMCMARCKFKAILVC