Protein Info for DVU0503 in Desulfovibrio vulgaris Hildenborough JW710

Name: pnp
Annotation: polyribonucleotide nucleotidyltransferase (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 760 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 14 to 697 (684 residues), 967.8 bits, see alignment E=1.7e-295 PF01138: RNase_PH" amino acids 17 to 147 (131 residues), 94.2 bits, see alignment E=2.2e-30 amino acids 331 to 462 (132 residues), 97.9 bits, see alignment E=1.6e-31 PF03725: RNase_PH_C" amino acids 150 to 213 (64 residues), 61.1 bits, see alignment E=2.1e-20 amino acids 466 to 534 (69 residues), 30 bits, see alignment E=1e-10 PF03726: PNPase" amino acids 245 to 327 (83 residues), 63.8 bits, see alignment E=4.4e-21 PF00013: KH_1" amino acids 563 to 619 (57 residues), 42.4 bits, see alignment 1.2e-14 PF00575: S1" amino acids 627 to 691 (65 residues), 37.8 bits, see alignment E=5.1e-13

Best Hits

Swiss-Prot: 100% identical to PNP_DESVH: Polyribonucleotide nucleotidyltransferase (pnp) from Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 100% identity to dvl:Dvul_2438)

MetaCyc: 49% identical to polynucleotide phosphorylase (Escherichia coli K-12 substr. MG1655)
Exoribonuclease II. [EC: 3.1.13.1]; Polyribonucleotide nucleotidyltransferase. [EC: 3.1.13.1, 2.7.7.8]

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.1

Use Curated BLAST to search for 2.7.7.8 or 3.1.13.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q72ER6 at UniProt or InterPro

Protein Sequence (760 amino acids)

>DVU0503 polyribonucleotide nucleotidyltransferase (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MTNIFNAMRVTATVGGKEIVFETGRLANQADGAVWIQCGGTVVLVTACSQATDRDLGFFP
LTVEYSEKMYAAGRIPGSFFRREIGRPSERETLVSRLIDRPIRPLFPKGLKDEVQVLANV
ISSDQNNDSDVLAVTGASTALGLSSIPFDGPVAGARIGRIDGQFVINPTIKEMERSDLNI
VLAASRDAVVMVEGEASFVPEAVIVEALEWGHKEIQPLIDAQLKLREMAGKAKREFTAPV
EDADLAARVAALATADLDVALRIPEKMARKDARKAVKEKVMEALVADPAYAEDTTPLRAV
GDILSALEKRIVRERIVREGRRIDGRDTTTVRPILIEAGILPRAHGSALFARGETKSLVV
ATLGSSTDEQRMDSLTGDVTKRFMLHYNFAPYCVGEVKPVRVSRREIGHGALAEKALRPI
LPLGEDFPFTLRVVAETMESNGSSSMAAVCGGCLSLMDAGVPITAPVAGVAMGLIKEGDQ
YVVLTDILGDEDALGDMDFKIAGTSEGITAVQMDIKVKGLPTDVMARAMQQARDARLHIL
GEMGKVLEAPRAELSAYAPQHAEVFVNPDIIRIIIGPGGKNIKAITATTGASIDIEDSGR
VSIFAPTLEAMEMAREMVQYYDQRADIGKNYTGKVRKVLEIGAIVEILPNLEALVHISQL
DTNRVEQASDVARLGEDMVVKVIEINGDRIRASRKAVLLEEQGIEWKPEDTARPSGPREG
GRRDGGRDGRRDGGRDGRRDGGRDGGRRDGGRRDGGRDRN