Protein Info for DVU0489 in Desulfovibrio vulgaris Hildenborough JW710

Name: paaK-1
Annotation: phenylacetate-coenzyme A ligase (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 45% identical to PAAK_ECOLI: Phenylacetate-coenzyme A ligase (paaK) from Escherichia coli (strain K12)

KEGG orthology group: K01912, phenylacetate-CoA ligase [EC: 6.2.1.30] (inferred from 100% identity to dvl:Dvul_2452)

MetaCyc: 43% identical to phenylacetate-CoA ligase (aerobic) (Aromatoleum evansii)
Phenylacetate--CoA ligase. [EC: 6.2.1.30]

Predicted SEED Role

"Phenylacetate-coenzyme A ligase (EC 6.2.1.30)" in subsystem Aromatic amino acid interconversions with aryl acids (EC 6.2.1.30)

MetaCyc Pathways

penicillin G and penicillin V biosynthesis (1/4 steps found)
phenylacetate degradation II (anaerobic) (1/4 steps found)
L-phenylalanine degradation IV (mammalian, via side chain) (3/9 steps found)
phenylacetate degradation I (aerobic) (1/9 steps found)
superpathway of phenylethylamine degradation (1/11 steps found)
anaerobic aromatic compound degradation (Thauera aromatica) (2/27 steps found)

KEGG Metabolic Maps

Phenylalanine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.30

Use Curated BLAST to search for 6.2.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q72ES9 at UniProt or InterPro

Protein Sequence (432 amino acids)

>DVU0489 phenylacetate-coenzyme A ligase (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MSQPYRFLPSLDEEKLHAIQLEGLRWTVRHAYEGSPRYRAKLRAAGVVPDDIVHLDDVRR
LPFTTVADLRDDYPLPLLSVPEKDVVRIHASSGTTGKRKILAYTQRDVDTFALQMARCYE
LAGLTQEDRVQIAVGYGLWTAGAGFQLGCERFGALTVPVGPGNLEMQLQLLTDLGVTCLC
STASMALLMAEEVERHGLRGDIRLRKVIFGAEAHSAKMRRTFEEKLGLEGSFDIAGMTEM
YGPGTGLECEAHDGIHYWADLFLVEILDPETLQPVEPGEVGEMVVTSLRKEASPLIRYRT
RDLTRLIPGTCSCGRNIPRHDHILGRSDDMIIFRGVNIYPGQIADVLHLFPEVGSEYHIE
LTRKEGRDHMLLKVERQPGAATGDERTLGVAIGNELHRKLMARVSVAVVAPGELPRSFAK
SKRVTDLRMVEG