Protein Info for DVU0405 in Desulfovibrio vulgaris Hildenborough JW710
Name: cobB-1
Annotation: cobyrinic acid a,c-diamide synthase (TIGR)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K02224, cobyrinic acid a,c-diamide synthase [EC: 6.3.1.- 6.3.5.9] (inferred from 100% identity to dvu:DVU0405)MetaCyc: 41% identical to siroamide synthase subunit (Allochromatium vinosum)
6.3.5.M1 [EC: 6.3.5.M1]
Predicted SEED Role
"Cobyrinic acid A,C-diamide synthase" in subsystem Cobalamin synthesis or Coenzyme B12 biosynthesis
MetaCyc Pathways
- adenosylcobalamin biosynthesis II (aerobic) (27/33 steps found)
- adenosylcobalamin biosynthesis I (anaerobic) (29/36 steps found)
- cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion) (13/15 steps found)
- cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation) (10/13 steps found)
- siroheme amide biosynthesis (1/2 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.3.1.-, 6.3.5.9
Use Curated BLAST to search for 6.3.1.- or 6.3.5.9 or 6.3.5.M1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q72F13 at UniProt or InterPro
Protein Sequence (468 amino acids)
>DVU0405 cobyrinic acid a,c-diamide synthase (TIGR) (Desulfovibrio vulgaris Hildenborough JW710) MSSARLVIAGLNGGTGKTILSLGTVRALTNAGHSVKPFKKGPDYIDAEWLGLAAGRAATN LDPFFLDAARLQSLFLHASAGASISIIEGNRGLYDGRDVTGSCSTAELARILDSPVVLAM DCTKMTRTAAAILSGVAAFEPGVKLAGVVLNRTANERHRSMLRQCIEHYTDIPVLGALPR LRDNPIPERHMGLVSDREFAQCAGELDRVAQFVAQHVDVERMAEIARSAQPLASNEPFWP VVAEGDGVRPRIGYVRDAALWFYYEENLEALRQAGADLVELRIIDGDPWPTLDGLYLGGG FPETLASELSACRERLAGIREAALAGLPVYAECGGFMVLGESIVMDGVEYPMSGVFPVQT VFHPKPQGLGYVEAEVVAENPFFPQGMVFRGHEFHYSACRPRDGGAVTHALRLNPGTGMG GGADGLVQANTFAAYTHIFGPAVQGWAERFVRAARGHRDQLNKGTDLS