Protein Info for DVU0085 in Desulfovibrio vulgaris Hildenborough JW710

Name: trpB-1
Annotation: tryptophan synthase, beta subunit (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 414 TIGR00263: tryptophan synthase, beta subunit" amino acids 16 to 398 (383 residues), 605.4 bits, see alignment E=2.2e-186 PF00291: PALP" amino acids 63 to 387 (325 residues), 148.3 bits, see alignment E=1.6e-47

Best Hits

Swiss-Prot: 64% identical to TRPB_FUSNN: Tryptophan synthase beta chain (trpB) from Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)

KEGG orthology group: K01696, tryptophan synthase beta chain [EC: 4.2.1.20] (inferred from 100% identity to dvl:Dvul_2876)

MetaCyc: 64% identical to tryptophan synthase beta subunit (Thermococcus kodakarensis)
Tryptophan synthase. [EC: 4.2.1.122, 4.2.1.20]

Predicted SEED Role

"Tryptophan synthase beta chain like (EC 4.2.1.20)" (EC 4.2.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.20

Use Curated BLAST to search for 4.2.1.122 or 4.2.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q72FX8 at UniProt or InterPro

Protein Sequence (414 amino acids)

>DVU0085 tryptophan synthase, beta subunit (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MTATATTLHRPDTSTGFFGAYGGQFVPDQLKPILDDLAATFDACKDDPSFIEEFTYYLTR
YSGRATPLFLCSNLTARLGGARIYLKREDLNHLGAHKVNNTIGQILLAKRMGKKKVIAET
GAGQHGVATAATAALMGMQCTIYMGAEDMERQKLNVFRMRMMGAEVVPAMSGQRTLKEAV
DEALAAWVQDANETFYLLGSAVGPHPYPAIVRHFQSVIGREARAQILEAEGRLPDCCIAC
VGGGSNAIGLFAEFVPDTSVRLIGVEPAGRGLTYGDHAATLCLGEPGVLHGFYSYMLKDD
KGEAAAVYSISAGLDYPGVGPEHSHLKDMNRAEYVSVTDVEAVDAFFTLSRAEGIIPALE
SSHALAHAMKMAPAMPKDAIIVVNLSGRGDKDVAQIEEMVSNGLVTPPAMVRGL