Protein Info for DVU3256 in Desulfovibrio vulgaris Hildenborough JW710

Name: mutM
Annotation: formamidopyrimidine-DNA glycosylase (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 365 PF01149: Fapy_DNA_glyco" amino acids 1 to 69 (69 residues), 66 bits, see alignment E=7.4e-22 amino acids 164 to 210 (47 residues), 38.3 bits, see alignment 2.8e-13 TIGR00577: DNA-formamidopyrimidine glycosylase" amino acids 162 to 364 (203 residues), 209.5 bits, see alignment E=3e-66 PF06831: H2TH" amino acids 227 to 314 (88 residues), 91.2 bits, see alignment E=5.2e-30 PF06827: zf-FPG_IleRS" amino acids 337 to 364 (28 residues), 36.7 bits, see alignment (E = 4.4e-13)

Best Hits

Swiss-Prot: 100% identical to FPG_DESVH: Formamidopyrimidine-DNA glycosylase (mutM) from Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303)

KEGG orthology group: K10563, formamidopyrimidine-DNA glycosylase [EC: 3.2.2.23 4.2.99.18] (inferred from 100% identity to dvu:DVU3256)

Predicted SEED Role

"Formamidopyrimidine-DNA glycosylase (EC 3.2.2.23)" in subsystem DNA Repair Base Excision (EC 3.2.2.23)

Isozymes

Compare fitness of predicted isozymes for: 4.2.99.18

Use Curated BLAST to search for 3.2.2.23 or 4.2.99.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q726D5 at UniProt or InterPro

Protein Sequence (365 amino acids)

>DVU3256 formamidopyrimidine-DNA glycosylase (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MPELPEVETIACGLRPALSGRRIVGVTVHNPGTLEGPLCTPAAFTEAVQGQRIADVGRRG
KLLLVAFASLPPVGHAGQPRPEGLSSSTVRDFLVTHGFHAAGCATSVHACAPLLADGQQT
RGRLAGHGDGMDGTSRTGSTLPGTGGTENSDAVAVADDDTVLGLAFHLKMTGRLFIHPPA
TPAGIHTRVVFDLEGGTRLFFDDARKFGYVRCITRRSLALWPFWRDLGPEPLETEARGFA
ARLARRRGRIKALLLDQKVVAGVGNIYADESLFRAGIRPDTQAHTLTPERLFALHGHLQD
VLRESIAECGSSIRDYRDAHGDAGAFQNSFRVYGRGGQPCRHCGTTLATAQVAGRTTVFC
PQCQR