Protein Info for DVU3216 in Desulfovibrio vulgaris Hildenborough JW710

Name: cckA
Annotation: sensor histidine kinase (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 565 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 276 to 301 (26 residues), see Phobius details PF02518: HATPase_c" amino acids 443 to 551 (109 residues), 109.2 bits, see alignment E=1.5e-35 PF14501: HATPase_c_5" amino acids 447 to 535 (89 residues), 23.2 bits, see alignment E=5.2e-09

Best Hits

KEGG orthology group: K02482, two-component system, NtrC family, sensor kinase [EC: 2.7.13.3] (inferred from 100% identity to dvu:DVU3216)

Predicted SEED Role

"Two-component sensor PilS" in subsystem Type IV pilus

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q726D7 at UniProt or InterPro

Protein Sequence (565 amino acids)

>DVU3216 sensor histidine kinase (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MEIGGYTRLRRRLMLAMLAFSLVPLLALGLFFQKQFTGTYNEKLVSGLEAVTESKRRALD
TFLEERVSQIKSLVYTHSFEELSNPARLGEIFSVIQANARSYVDIGIILSNGTHAAYVGP
YNLEGANYMDEPWFQEVMLKGQYVSDVFLGFRKYPHFVIAVARREGARTWIVRATIDSAA
INQLLRRSYSGNNSDAFLLNTEGLLQSDSRNNGEIMSHSAIPLPPRGKRGILIEPTHSAD
GRPMIAAMTWLERMPWLLVVMEDPREPLSPLERTNLLLVLFVMGGVAVICLGTFLTTRAI
VGKLKDSDRKQAMLDAEFLQSSKMAALGKLAAGVAHEVNNPLMLIRENAGWIKDLLVEED
PAAMRNHKELVTAADKIEMHVDRAKGVTHRMLGLARRLEPLQDDVCVNLLTEQTIKFLET
EALHRHITLVKELADDLPPITTDATQVQQVLLNILDNAIDAVGRDGTITIRTGAAPDRSE
VFVSVSDTGEGIPPELLSRIFDPFYTTKKVGEGTGLGLAICHSILEKLGGRIHAASAPGQ
GATFTVTLPLAANPALAADGMLNLR