Protein Info for DVU3214 in Desulfovibrio vulgaris Hildenborough JW710

Annotation: phosphoenolpyruvate synthase-related protein (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01326: PPDK_N
  • amino acids 139 to 445 (307 residues), 287.3 bits, E=1.7e-89
• PF00391: PEP-utilizers
  • amino acids 498 to 568 (71 residues), 56.1 bits, E=2.2e-19

Best Hits

KEGG orthology group: K01007, pyruvate, water dikinase [EC: 2.7.9.2] (inferred from 100% identity to dvu:DVU3214)

Predicted SEED Role

"Phosphoenolpyruvate synthase (EC 2.7.9.2)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.2)

MetaCyc Pathways

• hexitol fermentation to lactate, formate, ethanol and acetate (16/19 steps found)
• glycolysis II (from fructose 6-phosphate) (10/11 steps found)
• glycolysis I (from glucose 6-phosphate) (11/13 steps found)
• superpathway of anaerobic sucrose degradation (15/19 steps found)
• gluconeogenesis I (10/13 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (12/17 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (18/26 steps found)
• superpathway of N-acetylneuraminate degradation (15/22 steps found)
• superpathway of hexitol degradation (bacteria) (11/18 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (22/56 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 2.7.9.2

Use Curated BLAST to search for 2.7.9.2

Sequence Analysis Tools

See Q726D9 at UniProt or InterPro

Protein Sequence (884 amino acids)

>DVU3214 phosphoenolpyruvate synthase-related protein (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MASLLTKLRALFDRPPDPVALAEAERLRAVYRERCGHFRHLLSANKTALEVMADMEETLR
GGRPFGLHYVRGASTRVTASVFQMVRALNSLTEDRYRDLYASFDAVRTAIEACLGSRHRS
ERGPLVLPIADISLDMADETGGKMANLGEIRRRLGISVPDGFVVTASGFQYFMAANGLQE
EIDRRIQAANAGRLDEVFALSSSIQSLILRAPVPDDLAAAITAEVERLAACHAGPLRLAV
RSSAVGEDALGASFAGQYRSELNVTPEDVLDTWREVVASKYGVTAMTYRHNRGIPDEDVA
MCVGGLVMVDALAGGVAYSRNPLDLRDNTVVINAVAGLPKAVVDGSWTPDVFVVSRDVPP
RLVRREIASKPGRFVCDPVEGVRLVELPEAERTLPSIDDALAVEIASLAAGFEVYYGEPQ
DIEWALAPDGQLVILQSRPLKEAVHEAPLDIAAAAGPDVPGGGGSAYPVLLAGGAAASPG
TGAGDVYVVRRDADLLGFPDGAVLVVERSLPRWAPLLGRAAAVVAEAGGTAGHLASVARE
YGVPALFGIEGCVAALEGAGTVTVDADNRRVHPGRIEALLERTPPRNLMQGSPVFETLQQ
VARLVVPLNLLDPESPSFAPGNCRTLHDITRFCHEKSVDALFGAHEGSPAEALAGKQLRA
GVKLQYWVIDIDDGFIDPVPGKVVDIANIRSLPMLYLWDGMVAVPWAGPPTLDAQGFFSV
VFESTANPDLEATAASPLANKNFLMVSADFCNLQARFGYHFCTVEAQAGDDAHGNYVSFH
FKGGAASPDRRVSRALLVAEVLEEQGFRVDVKEDALFASCEGQPREETLALTRLLGYLII
HTRQVDMIMHNATARAALHQRLRDEMLAVRQRPAVVVPYVQPHR