Protein Info for DVU2942 in Desulfovibrio vulgaris Hildenborough JW710

Name: purB
Annotation: adenylosuccinate lyase (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00928: adenylosuccinate lyase
  • amino acids 1 to 429 (429 residues), 510 bits, E=2.6e-157
• PF00206: Lyase_1
  • amino acids 6 to 282 (277 residues), 177 bits, E=6.5e-56
• PF10397: ADSL_C
  • amino acids 350 to 428 (79 residues), 88.3 bits, E=3.4e-29

Best Hits

Swiss-Prot: 54% identical to PUR8_BACSU: Adenylosuccinate lyase (purB) from Bacillus subtilis (strain 168)

KEGG orthology group: K01756, adenylosuccinate lyase [EC: 4.3.2.2] (inferred from 100% identity to dvl:Dvul_0426)

MetaCyc: 40% identical to adenylosuccinate lyase (Methanocaldococcus jannaschii)
Adenylosuccinate lyase. [EC: 4.3.2.2]; 4.3.2.2 [EC: 4.3.2.2]; 4.3.2.- [EC: 4.3.2.2]

Predicted SEED Role

"Adenylosuccinate lyase (EC 4.3.2.2)" in subsystem De Novo Purine Biosynthesis or Purine conversions (EC 4.3.2.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (42/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (23/26 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• inosine-5'-phosphate biosynthesis II (5/5 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (6/7 steps found)
• adenosine ribonucleotides de novo biosynthesis (3/3 steps found)
• inosine-5'-phosphate biosynthesis I (5/6 steps found)
• inosine-5'-phosphate biosynthesis III (4/6 steps found)
• dZTP biosynthesis (3/5 steps found)
• tetrahydromethanopterin biosynthesis (2/14 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Biosynthesis of alkaloids derived from histidine and purine
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.2.2

Sequence Analysis Tools

See Q727B3 at UniProt or InterPro

Protein Sequence (430 amino acids)

>DVU2942 adenylosuccinate lyase (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MIDRYTRPEMGNIWTLDNRFRAWLEVEIAVCEAWHRLGVIDAESMQTIRDKADFETDRIL
EIEQVTRHDVIAFLTAVEEKVGPAARFIHLGCTSSDIVDTANGLLLVRAGELIVKGFERL
LDVIGKVAHANKGRLCMGRTHGIHAEPTSFGLKMACFHAEFQRHLARFKDALEGIRVGKI
SGAVGTYAFLSPEMEAIALDLIGLKVDPVSTQIIQRDRHAHYFTSLALAAGGVERLCVEL
RHLQRTEVLEAEEGFAKGQKGSSAMPHKKNPISAENMTGLARLIRTNALASMENMALWHE
RDISHSSVERVIMPDSTILMDYVLHRLANLVENLRIIPENMDRNLAMSYGLFYSQRVLLT
LVESGMARQEAYVMVQRCAMQSWETHRPFPDIVRADADISSRIDAATLDALFDPSYYLRH
EDTIFRRVFA