Protein Info for DVU2254 in Desulfovibrio vulgaris Hildenborough JW710
Name: thyX
Annotation: thymidylate synthase ThyX (TIGR)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to THYX_DESVH: Flavin-dependent thymidylate synthase (thyX) from Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303)
KEGG orthology group: K03465, thymidylate synthase (FAD) [EC: 2.1.1.148] (inferred from 100% identity to dvl:Dvul_0990)Predicted SEED Role
"Thymidylate synthase thyX (EC 2.1.1.-)" in subsystem Folate Biosynthesis (EC 2.1.1.-)
MetaCyc Pathways
- pyrimidine deoxyribonucleotides de novo biosynthesis III (9/9 steps found)
KEGG Metabolic Maps
- Alkaloid biosynthesis I
- Anthocyanin biosynthesis
- Benzoxazinone biosynthesis
- Biosynthesis of alkaloids derived from shikimate pathway
- Carotenoid biosynthesis - General
- Flavonoid biosynthesis
- Histidine metabolism
- Insect hormone biosynthesis
- Naphthalene and anthracene degradation
- One carbon pool by folate
- Phenylpropanoid biosynthesis
- Porphyrin and chlorophyll metabolism
- Pyrimidine metabolism
- Tryptophan metabolism
- Tyrosine metabolism
- Ubiquinone and menaquinone biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 2.1.1.-
Use Curated BLAST to search for 2.1.1.- or 2.1.1.148
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q729U4 at UniProt or InterPro
Protein Sequence (245 amino acids)
>DVU2254 thymidylate synthase ThyX (TIGR) (Desulfovibrio vulgaris Hildenborough JW710) MPRTEPRVELLAHTPEPLSLLYAAFRQCYHAGFVADMWPRLLSGEIEREKQGAFIASILE SGHSSPIEHVSFTFAIEGVSRALTHQLVRHRIASFSQQSQRYVDGSHFDYVMPPAIARNA AAKARFEQFMEDVGSAYRDIKALLEQDGRTGSRANEDARFVLPQAAASKIVVTMNCRALV HFFEERCCMRAQWEIRAVADVMLGLCREVLPELFAHAGAKCERLGYCPEGERFTCGRYPL RQSMP