Protein Info for DVU2250 in Desulfovibrio vulgaris Hildenborough JW710

Annotation: AMP-binding protein (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 33 to 407 (375 residues), 239.7 bits, E=4.9e-75
• PF13193: AMP-binding_C
  • amino acids 458 to 536 (79 residues), 82.4 bits, E=3.5e-27

Best Hits

KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 100% identity to dvu:DVU2250)

Predicted SEED Role

"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)

MetaCyc Pathways

• superpathway of acetate utilization and formation (3/3 steps found)
• acetate and ATP formation from acetyl-CoA III (1/1 steps found)
• acetate conversion to acetyl-CoA (1/1 steps found)
• L-isoleucine biosynthesis V (2/3 steps found)
• ethanol degradation II (2/3 steps found)
• ethanol degradation IV (2/3 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• chitin deacetylation (2/4 steps found)
• ethanol degradation III (1/3 steps found)
• adlupulone and adhumulone biosynthesis (1/6 steps found)
• colupulone and cohumulone biosynthesis (1/6 steps found)
• lupulone and humulone biosynthesis (1/6 steps found)
• cis-geranyl-CoA degradation (1/9 steps found)
• superpathway of bitter acids biosynthesis (3/18 steps found)

KEGG Metabolic Maps

• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.1

Use Curated BLAST to search for 6.2.1.1

Sequence Analysis Tools

See Q729U8 at UniProt or InterPro

Protein Sequence (551 amino acids)

>DVU2250 AMP-binding protein (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MDKLTFASYDEFLRVFSLDAPETFNFAFDVLDAMANETPDRLAIAHVDDAGVRRDYTFAW
LADASARLANALKARGVRKGHRVMLVLHRRIEFWVSMLALHRLGAVAIPAPAQLTPKDIV
FRVERAQVRAVIVDDGITDRIEAARPDCPTLSVLVQCGGTPLPDGWCDYEALCADASPSF
PRPTAPDELACGEDPLLIFFSSGTTGMPKMVEHVHTYPLGHLVTGMYWHDLRPGDLHLTV
ADTGWGKAVWGKFYGQWMAGAAVFVYDFRGKFDPEALLDVVAKNGVTTFCAPPTVYRFLV
RADLSRYDLSKLRHCTTAGELLNESVFHGWKAATGLSIYEGYGQTETTLQIATLSCMEAK
PGSIGRPMPGWGITLLDHEGKECPTGEEGEICIRISDGLPVGLFRGYVEDAEKTASVMFD
GYYHTGDKAWMDEDGYLWFLGRVDDLIKSSGYRIGPFEVESALVAHPAVVEAAVTGVPDP
LRGQAVKATIVLAAGYEAGEVLTKELQDHVKKVTAPYKYPRIVEYVAELPKTISGKIKRA
EIRQRDSEKSA