Protein Info for DVU1871 in Desulfovibrio vulgaris Hildenborough JW710

Name: aspA
Annotation: aspartate ammonia-lyase (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 60% identical to ASPA_PSEAE: Aspartate ammonia-lyase (aspA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01744, aspartate ammonia-lyase [EC: 4.3.1.1] (inferred from 100% identity to dvl:Dvul_1293)

MetaCyc: 54% identical to aspartate ammonia-lyase (Escherichia coli K-12 substr. MG1655)
Aspartate ammonia-lyase. [EC: 4.3.1.1]

Predicted SEED Role

"Aspartate ammonia-lyase (EC 4.3.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 4.3.1.1)

MetaCyc Pathways

L-glutamate degradation II (2/2 steps found)
superpathway of L-aspartate and L-asparagine biosynthesis (3/4 steps found)
L-aspartate degradation II (aerobic) (2/3 steps found)
L-aspartate degradation III (anaerobic) (2/3 steps found)
L-canavanine degradation II (1/6 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.1

Use Curated BLAST to search for 4.3.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q72AW9 at UniProt or InterPro

Protein Sequence (468 amino acids)

>DVU1871 aspartate ammonia-lyase (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MEYRTERDSLGEKRVPAGVYYGVQTLRALENFQITAMPVATFPRFIQALAAVKKAAALAN
MELGLLDAGVGEAIVNACRDVRAGRFDDQFPVDVIQGGAGTSVNMNANEVIANRALELLG
HPKGRYEVISPLNHVNLSQSTNDVYPTALRIALVWYARELSTALRDLRAAFDAKGDEFAG
VIKMARTQLQDAVPITLGAEFAAWGVTIGEDIDRLDDAARLLCEVNIGATAVGTGINSVP
GYASLVRERLADICGLPLVTAANLVEATSDAGAYVMLSGLLKRVAVKLSKICNDLRLLAS
GPFTGLHEINLPPMQPGSSIMPGKVNPVIPEVVNQVCYQVIGNDLTVTFAAEGGQLELNV
FLPVIALNLFQSLQMLTKASRTLRERCVVGITANPERCLELVRHSLGVVTALAPVIGYET
AARLAKEAQESGRSVPEVLQAEGIMTPEEFEELLDPAKMLSPRDVRRR