Protein Info for DVU1772 in Desulfovibrio vulgaris Hildenborough JW710

Name: gltD
Annotation: pyridine nucleotide-disulfide oxidoreductase (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF10589: NADH_4Fe-4S
  • amino acids 63 to 143 (81 residues), 67.6 bits, E=2.6e-22
• PF14691: Fer4_20
  • amino acids 161 to 238 (78 residues), 61.4 bits, E=2.5e-20
• PF07992: Pyr_redox_2
  • amino acids 259 to 571 (313 residues), 104.4 bits, E=2.7e-33
• PF13450: NAD_binding_8
  • amino acids 262 to 298 (37 residues), 33.2 bits, 1.8e-11
• PF01593: Amino_oxidase
  • amino acids 268 to 294 (27 residues), 21.6 bits, see alignment (E = 4.4e-08)
• PF00070: Pyr_redox
  • amino acids 397 to 462 (66 residues), 22.4 bits, E=5.1e-08

Best Hits

KEGG orthology group: K15022, formate dehydrogenase beta subunit [EC: 1.2.1.43] (inferred from 100% identity to dvu:DVU1772)

Predicted SEED Role

"Glutamate synthase [NADPH] small chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)

MetaCyc Pathways

• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)
• superpathway of L-citrulline metabolism (7/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Methane metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13

Use Curated BLAST to search for 1.2.1.43 or 1.4.1.13

Sequence Analysis Tools

See Q72B66 at UniProt or InterPro

Protein Sequence (673 amino acids)

>DVU1772 pyridine nucleotide-disulfide oxidoreductase (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MARVIYGIWDGTVHDNRGKALFEIPDAPGFEGFDTFNQGNSIRAFVGDRGFFVFEPGVNL
LDALWQYMERAARESCGKCTPCRVGTGIIRDSLAALLRDGVGSGGLGTLVELAEQVRSTS
LCGLGRTCTVALCGALEHFQDMLVEGARTASGQQHGMSYVTAPCIEACPARVNVPRYIDY
IRDGMPSHSLGVILQKYPMAATCGRVCVRFCEQACRRGLVDEPVGIKILKRYVADHESHL
AQGWFSPDIVRDRKPGHLKVAVVGAGPAGISCAYHLLLNGYPVDVFEARNEAGGMAACGI
PSYRLPKDVLRTETDIVERLGGRFFYGQRLGHDVSLDGLREQGYKAVFLALGCPEGRLLG
IEGEDTSLEGYQPGIDFLLRVHDHVESQADASLSGVVVVVGGGNVAMDCVRSALRLGARE
VHLVYRRTRDAMPADHEEIEAAEKEGVVFHYLTNPVRIITRQMVDSAGVTRDRVVGVEVV
DMREGEPDARGRRGVVAVSGSGRVIPCDTLIPAIGQQMDSTALSPEDGVDVDARGRVVSD
QTTLATTRPGVFAGGDCALGPSTLIQAMATGLKGARSIDDYLRYGRVRFFPRSRMRRIIR
DFKPLSQQWVDTPVRHLYRIHAQELDPAYRRQLFDEVECPISAEDAYHEAGRCLRCYRVY
SVITEAAIPQCDD