Protein Info for DVU1608 in Desulfovibrio vulgaris Hildenborough JW710

Name: ligA
Annotation: DNA ligase, NAD-dependent (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 799 PF01653: DNA_ligase_aden" amino acids 38 to 354 (317 residues), 365.4 bits, see alignment E=9.7e-113 TIGR00575: DNA ligase, NAD-dependent" amino acids 46 to 706 (661 residues), 755.8 bits, see alignment E=2e-231 PF03120: DNA_ligase_OB" amino acids 359 to 435 (77 residues), 113.1 bits, see alignment E=1.9e-36 PF03119: DNA_ligase_ZBD" amino acids 444 to 470 (27 residues), 41.1 bits, see alignment (E = 5.4e-14) PF12826: HHH_2" amino acids 547 to 609 (63 residues), 84.7 bits, see alignment 1.6e-27 PF14520: HHH_5" amino acids 552 to 598 (47 residues), 32.1 bits, see alignment 5.3e-11 PF00533: BRCT" amino acids 637 to 708 (72 residues), 35.4 bits, see alignment E=4.2e-12

Best Hits

Swiss-Prot: 100% identical to DNLJ_DESVH: DNA ligase (ligA) from Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303)

KEGG orthology group: K01972, DNA ligase (NAD+) [EC: 6.5.1.2] (inferred from 100% identity to dvu:DVU1608)

MetaCyc: 48% identical to DNA ligase (Escherichia coli K-12 substr. MG1655)
DNA ligase (NAD(+)). [EC: 6.5.1.2, 6.5.1.6]

Predicted SEED Role

"DNA ligase (EC 6.5.1.2)" in subsystem DNA Repair Base Excision (EC 6.5.1.2)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.5.1.2 or 6.5.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q72BM7 at UniProt or InterPro

Protein Sequence (799 amino acids)

>DVU1608 DNA ligase, NAD-dependent (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MTEVKTGRVVDDAPVNDAPENNAAEATSPARHDAIPEEARQRAAQLRADLERHNRLYYEL
DTPEISDAEYDALYRELVGLETRWPALRDEASPTQRVGGEVLEGLEKQAHTLRMYSLDNA
FSRDEWGAFIQRMYNALPDAPSAFWCDPKMDGLALEVIYENGVFTSALTRGNGEVGEVVT
AAMRTVRNLPLALRGDDVPRRIEVRGEVVIAKADFEQLNARQSAAGGKLFANPRNAAAGS
VRQLDTTVTAGRPLQFLAYGVGQVVLEGGTAPWTTHSGLMARLREWGFDTPPEGRLCASP
DEVWAYYEALGARRESLAIEIDGVVAKLDDTEAQEALGFTARAPRWALALKFPAMQARTR
LDDIRVQVGRTGVLTPVAILEPVRVGGVEVSRATLHNEDEIRAKGLMLGDMVLVQRAGDV
IPEVVRPLVEERTGDERPFVFPENCPECDSPVVRPQGEVAHRCVNVMCPAVRRQSIIHFV
SKAGLDVRGVGERWVQQLVDGGHVTSPVGLFLLTKLDLMRFERMGPTSAANFVTALDAAR
TGATLVRLICALGIRHVGEQTARTLAANFTDLDALREADAETLQQLPDIGPEVAGSIRSF
FANEGNLELLERLRAIGLWPKRQDAPPASEGADAIVLPLQGLKVLFTGSLTRVGRTEAED
MARAAGANIASSVTKSLDLLVVGGKPGSKLEKARKLGIRVMEEADFFAMLASGVASVDAS
EAVAEETPPSQEAAGAEDAPSQGAAHVRTASDETGSASGDDSRGAAAENDPARPARGGAM
STAEGEDVPRGRAEQLKLF