Protein Info for DVU1089 in Desulfovibrio vulgaris Hildenborough JW710

Name: alaS
Annotation: alanyl-tRNA synthetase (TIGR)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00344: alanine--tRNA ligase
  • amino acids 6 to 860 (855 residues), 975.6 bits, E=1.3e-297
• PF01411: tRNA-synt_2c
  • amino acids 6 to 559 (554 residues), 711 bits, E=1.5e-217
• PF07973: tRNA_SAD
  • amino acids 659 to 702 (44 residues), 56.2 bits, 4.1e-19
• PF02272: DHHA1
  • amino acids 738 to 877 (140 residues), 84.9 bits, E=1.1e-27

Best Hits

Swiss-Prot: 100% identical to SYA_DESVH: Alanine--tRNA ligase (alaS) from Desulfovibrio vulgaris (strain Hildenborough / ATCC 29579 / DSM 644 / NCIMB 8303)

KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 100% identity to dvl:Dvul_1906)

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

• tRNA charging (21/21 steps found)
• shinorine biosynthesis (3/8 steps found)
• vanchrobactin biosynthesis (2/8 steps found)
• enterobactin biosynthesis (4/11 steps found)
• ergotamine biosynthesis (4/12 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• bacillibactin biosynthesis (3/12 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• superpathway of chorismate metabolism (31/59 steps found)
• phosalacine biosynthesis (1/25 steps found)
• phosphinothricin tripeptide biosynthesis (1/25 steps found)
• corallopyronin A biosynthesis (2/30 steps found)
• colibactin biosynthesis (7/38 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.7

Sequence Analysis Tools

See P61700 at UniProt or InterPro

Protein Sequence (879 amino acids)

>DVU1089 alanyl-tRNA synthetase (TIGR) (Desulfovibrio vulgaris Hildenborough JW710)
MITANEIRRRFLEFYKAHGHEAVRSSSLVPKDDPSLLFTNAGMVQFKKIFLGQEKRAYSR
ATTSQKCLRVGGKHNDLENVGRTARHHTFFEMLGNFSFGDYFKEDAIKFAWKFLTEELKL
PKERLYATVFRDDDEAEQLWLKHSDIPAERIYRMGEKDNFWSMGDTGPCGPCSEILIDQG
EHMTCGPDCGIGKCDCDRFLEIWNLVFMQYDQDATGKREPLPKPSIDTGMGLERITAVCQ
GVFSNFDTDIFQAIIQYTCGLANVSYRSDDETDTALRVIADHSRAIAFMITDGILPSNEG
RGYVLRRLIRRAYRFGRLIGLTDTFLHKTALKVVDIMGDDYPELRENSDFMARVVREEED
RFNRTLDKGLSMLEDELATLSASGAAHVPGDIAFRLYDTFGFPLDIVNDIAEKRGFSVDE
DGFKALMKEQKERAKAAWKGSGEKDIASRFQPLLEEGMRSEFIGYDHLCGEGRIVALMDE
HALAVERLAAGQKGYLVTNRTPFYGASGGQSGDIGTIASPSGKVRVVDTIKPSPELVVHH
VEAVEGDILLDQEVDLTVTEDDRVASARNHTCTHLLHAALRRVLGDHVKQAGSLVAPDRL
RFDFTHIAPMTPEELAAVEREVNRVIMADIPLETDHMHYDDAVKRGAMALFGEKYGDEVR
VVAIADESVELCGGTHLRATGQAGLFLIVSESGVAAGVRRIEALTGWNAMHAVLAQRSEQ
AQLSAMLKARPGEIVSRVESLQKENRTLRKDMERAAAQATSGQGRNIMDEATDVAGVKLL
AAKVEVPNVKALRDLMDDVRSKLVSGIACLAAVDGDKVQLIIAVSKDLQTRFTAPQLIKD
VAAEVGGSGGGRPDMAQAGGTNPAGIDAAFAKLRSLIGG