Protein Info for SO4745 in Shewanella oneidensis MR-1

Name: glmU
Annotation: UDP-N-acetylglucosamine pyrophosphorylase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01128: IspD
  • amino acids 4 to 134 (131 residues), 32.9 bits, E=1.4e-11
• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 4 to 451 (448 residues), 636 bits, E=1.8e-195
• PF12804: NTP_transf_3
  • amino acids 5 to 128 (124 residues), 74.8 bits, E=2.3e-24
• PF00483: NTP_transferase
  • amino acids 5 to 205 (201 residues), 51.6 bits, E=2.6e-17
• PF14602: Hexapep_2
  • amino acids 281 to 304 (24 residues), 12.1 bits, see alignment (E = 3.5e-05)
  • amino acids 392 to 426 (35 residues), 30.9 bits, 4.6e-11
• PF00132: Hexapep
  • amino acids 392 to 426 (35 residues), 35.5 bits, 1.4e-12

Best Hits

Swiss-Prot: 100% identical to GLMU_SHEON: Bifunctional protein GlmU (glmU) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 100% identity to son:SO_4745)

MetaCyc: 63% identical to fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylglucosamine diphosphorylase. [EC: 2.7.7.23]; Glucosamine-1-phosphate N-acetyltransferase. [EC: 2.7.7.23, 2.3.1.157]

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• O-antigen building blocks biosynthesis (E. coli) (10/11 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• peptidoglycan recycling I (9/14 steps found)
• chitin biosynthesis (5/9 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (9/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Sequence Analysis Tools

See Q8E8C2 at UniProt or InterPro

Protein Sequence (454 amino acids)

>SO4745 UDP-N-acetylglucosamine pyrophosphorylase (NCBI ptt file) (Shewanella oneidensis MR-1)
MALNVVILAAGKGTRMRSDLPKVLHPIAHKSMVQHVIDTAHSIGSDAIQLVYGYGADKLK
STLGEQKLNWILQAEQLGTGHAVAQAIPNIDDNDTVLILYGDVPLIQASTLEALLAARPE
YGVAILTVNLANPMGYGRIVREQGKVVGIVEQKDANPEQLAINEVNTGIMAVPGNALKTW
LDRLSNNNAQGEYYLTDIIAMAHADGVEINTAQPQSAIEVEGANNRVQLAQLERAYQARE
AEKLMIAGANLRDPSRIDIRGEVTVGMDVMIDVNVIFEGKVVIGNNVTIGAGAIIIDTEI
ADNAEIKPYSIIEGAKLGVAASAGPFARLRPGAELMQDAHIGNFVEMKKAVLGVGSKAGH
LAYLGDAQIGAGVNIGAGTITCNYDGANKHLTVIEDNVFVGSDTQLVAPVTIGKGATLGA
GSTITRDVGEDELVITRVKQKHLTGWQRPVKIKK