Protein Info for SO4478 in Shewanella oneidensis MR-1

Name: cpxA
Annotation: sensor protein CpxA (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 449 transmembrane" amino acids 6 to 30 (25 residues), see Phobius details amino acids 160 to 181 (22 residues), see Phobius details PF16527: CpxA_peri" amino acids 43 to 143 (101 residues), 30.3 bits, see alignment E=1.2e-10 PF00672: HAMP" amino acids 176 to 230 (55 residues), 54.5 bits, see alignment 3e-18 PF00512: HisKA" amino acids 236 to 294 (59 residues), 48.2 bits, see alignment E=2.3e-16 PF02518: HATPase_c" amino acids 343 to 448 (106 residues), 83.2 bits, see alignment E=4.7e-27 PF13589: HATPase_c_3" amino acids 346 to 418 (73 residues), 26.8 bits, see alignment E=1.2e-09

Best Hits

KEGG orthology group: K07640, two-component system, OmpR family, sensor histidine kinase CpxA [EC: 2.7.13.3] (inferred from 100% identity to son:SO_4478)

Predicted SEED Role

"Copper sensory histidine kinase CpxA" in subsystem Orphan regulatory proteins

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8E917 at UniProt or InterPro

Protein Sequence (449 amino acids)

>SO4478 sensor protein CpxA (NCBI ptt file) (Shewanella oneidensis MR-1)
MPNRLFIKLLLGFWLCSSLIIALVGLLPLLQQNHDRAPIPPHLQKVLATIAQRIQENPTL
LKSDFLRRWERHRDMEGKPLRLYLTNSQGQVINTNRISRGVRSFMLMADEEKQPISHQFK
DELVFGPYQFTLEGETYFLYGRLPDTHPRPWFFFFIENKLLTLSLAILLSGLLCALLAWH
LGKPLRSLKKSADALAEGDLSNRVDKTTTQRNDEIGQLATAFNSMADSIEAMVKNQQRLM
GDISHELRTPLTRLQLSLALARKKGQQTTETDRIAYEAEQLEQLIAELLELSRVKLSTNE
TKVCLGLAESLSQVLDDAEFEADQQGKKITIDIDEAIELSHYPKSLSRAIENLLRNAIRY
AKSDIHLHASQTSGQVQITIKDDGPGIDPAELESIFKPFYRPDSARQRESGGWGLGLAIT
EAAISAHKGKIKAENREPHGLEVNISLPT