Protein Info for SO4349 in Shewanella oneidensis MR-1

Name: ilvC
Annotation: ketol-acid reductoisomerase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF07991: IlvN
  • amino acids 35 to 204 (170 residues), 187.1 bits, E=1.9e-59
• TIGR00465: ketol-acid reductoisomerase
  • amino acids 36 to 367 (332 residues), 401.1 bits, E=1.4e-124
• PF01450: IlvC
  • amino acids 210 to 341 (132 residues), 92.9 bits, E=2.4e-30
  • amino acids 360 to 482 (123 residues), 45.5 bits, E=9.5e-16

Best Hits

Swiss-Prot: 100% identical to ILVC_SHEON: Ketol-acid reductoisomerase (NADP(+)) (ilvC) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K00053, ketol-acid reductoisomerase [EC: 1.1.1.86] (inferred from 100% identity to son:SO_4349)

MetaCyc: 73% identical to ketol-acid reductoisomerase (NADP+) (Escherichia coli K-12 substr. MG1655)
Ketol-acid reductoisomerase. [EC: 1.1.1.86]; 1.1.1.86 [EC: 1.1.1.86]; 2-dehydropantoate 2-reductase. [EC: 1.1.1.86, 1.1.1.169]

Predicted SEED Role

"Ketol-acid reductoisomerase (EC 1.1.1.86)" in subsystem Branched-Chain Amino Acid Biosynthesis or Coenzyme A Biosynthesis (EC 1.1.1.86)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• superpathway of L-threonine metabolism (15/18 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• superpathway of coenzyme A biosynthesis I (bacteria) (8/9 steps found)
• L-valine biosynthesis (4/4 steps found)
• phosphopantothenate biosynthesis I (4/4 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (8/10 steps found)
• pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
• phosphopantothenate biosynthesis III (archaea) (2/4 steps found)
• L-isoleucine biosynthesis III (4/7 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Pantothenate and CoA biosynthesis
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.169

Use Curated BLAST to search for 1.1.1.169 or 1.1.1.86

Sequence Analysis Tools

See Q8E9D5 at UniProt or InterPro

Protein Sequence (492 amino acids)

>SO4349 ketol-acid reductoisomerase (NCBI ptt file) (Shewanella oneidensis MR-1)
MANYFNSLNLRQQLAQLGQCRFMDRSEFSDGCNYIKDWNIVILGCGAQGLNQGLNMRDSG
LNIAYALRPEAIAQKRASWQKATDNGFKVGTFEELIPSADLVLNLTPDKQHSNVVSAVMP
LMKQGATLSYSHGFNIVEEGMQIRPDITVIMVAPKCPGTEVREEYKRGFGVPTLIAVHPE
NDPNGDGLDIAKAYASATGGDRAGVLQSSFIAEVKSDLMGEQTILCGMLQTGAILGYEKM
VADGVEPGYAAKLIQQGWETVTEALKHGGITNMMDRLSNPAKIKAFEIAEDLKDILQPLF
EKHMDDIISGEFSRTMMQDWANDDANLLRWRAETGETGFENAPVSSEHIDEQTYFDKGIF
LVAMIKAGVELAFDTMVSAGIVEESAYYESLHETPLIANTIARKRLYEMNVVISDTAEYG
CYLFNHAAVPLLRDYVNAMSPEYLGAGLKDSSNSVDNLQLIAINDAIRHTAVEYVGAELR
GYMTDMKSIVGA