Protein Info for SO4230 in Shewanella oneidensis MR-1

Name: glpK
Annotation: glycerol kinase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 494 TIGR01311: glycerol kinase" amino acids 4 to 493 (490 residues), 741.7 bits, see alignment E=1.5e-227 PF00370: FGGY_N" amino acids 5 to 251 (247 residues), 278 bits, see alignment E=7.8e-87 PF02782: FGGY_C" amino acids 261 to 449 (189 residues), 163.3 bits, see alignment E=6.8e-52

Best Hits

Swiss-Prot: 100% identical to GLPK_SHEON: Glycerol kinase (glpK) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K00864, glycerol kinase [EC: 2.7.1.30] (inferred from 100% identity to son:SO_4230)

MetaCyc: 72% identical to glycerol kinase (Escherichia coli K-12 substr. MG1655)
Glycerol kinase. [EC: 2.7.1.30]

Predicted SEED Role

"Glycerol kinase (EC 2.7.1.30)" in subsystem Glycerol and Glycerol-3-phosphate Uptake and Utilization or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or MLST (EC 2.7.1.30)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.30

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8E9N7 at UniProt or InterPro

Protein Sequence (494 amino acids)

>SO4230 glycerol kinase (NCBI ptt file) (Shewanella oneidensis MR-1)
MQKKYVVALDQGTTSSRAIVFDHDANIVSVSQREFTQLYPNPGWVEHDPMEIWASQSSVL
IESLARAGIHSDAVAAIGITNQRETTIIWEKATGKPVYNAIVWQCRRSAEICEQLKAQGL
EDYVRQNTGLLLDPYFSGTKIKWILDNVPDARAKAKRGELLFGTVDTWLLWKLTEGKVHV
TDPTNAARTLLFNIHSLSWDSKLLEALDIPAAMLPEVKPSCSVYGTTRIAGEGSEIPLAG
IAGDQQAALFGQLCVEQGMAKNTYGTGCFLLMNTGSKAVRSSHGLLTTVAVGAQGEVNYA
LEGSVFMGGATIQWLRDELGLIRDASDTEYFASKVADTNGVYLVPAFVGLGAPYWDPNAR
GALFGLTRGANRNHIIRAALESIAYQSKDLLDAMIKDSGVSLKRLKVDGGAVANDFLMQF
QADITDVEVLRPSVCETTALGAAFLAGLAVGFWNSVTELEHKACIDTHFKPSINADYRQQ
LYAGWQDAVARTRS