Protein Info for SO3599 in Shewanella oneidensis MR-1

Name: cysP
Annotation: sulfate ABC transporter, periplasmic sulfate-binding protein (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 335 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details TIGR00971: sulfate ABC transporter, sulfate-binding protein" amino acids 18 to 332 (315 residues), 451.9 bits, see alignment E=5.9e-140 PF13531: SBP_bac_11" amino acids 40 to 281 (242 residues), 125.1 bits, see alignment E=3.9e-40 PF01547: SBP_bac_1" amino acids 41 to 277 (237 residues), 56.6 bits, see alignment E=4.5e-19

Best Hits

Swiss-Prot: 60% identical to CYSP_ECOLI: Thiosulfate-binding protein (cysP) from Escherichia coli (strain K12)

KEGG orthology group: K02048, sulfate transport system substrate-binding protein (inferred from 100% identity to son:SO_3599)

MetaCyc: 60% identical to thiosulfate/sulfate ABC transporter periplasmic binding protein CysP (Escherichia coli K-12 substr. MG1655)
ABC-19-RXN [EC: 7.3.2.5]; ABC-7-RXN [EC: 7.3.2.5, 7.3.2.3]; 7.3.2.3 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-478 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-479 [EC: 7.3.2.5, 7.3.2.3]

Predicted SEED Role

"Sulfate and thiosulfate binding protein CysP" in subsystem Cysteine Biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.3 or 7.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EBC6 at UniProt or InterPro

Protein Sequence (335 amino acids)

>SO3599 sulfate ABC transporter, periplasmic sulfate-binding protein (NCBI ptt file) (Shewanella oneidensis MR-1)
MPVKLTKALLGTLLLGASLNVAAADQTLLNSSYDISRELFNAYNPVFAKHWQEKTGKTVE
IKQSHAGSSAQARSILQGLPADVVTFNQVTDVQILHDRGKLIPENWQQLLPNASSPYYST
IAFLVRKGNPKQISDWGDLAKDDVKLVFPNPKTSGNARYTYLAALGFAQKNYGKDNEASL
DAFLKKFLGNVAVFDTGGRGATTSFVERGIGDVLITFESEVNNIRQQYGADGYQVVVPKT
SILAEFPVAVVEKNAKRNGTQELATEYLSYLYSEESQRLLAGFNYRVHNEKVIAEFTKQF
PTVELMTVEQIIGNWDNAMKTQFANGAKLDQLLKR