Protein Info for SO3308 in Shewanella oneidensis MR-1

Name: engA
Annotation: GTP-binding protein EngA (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03594: ribosome-associated GTPase EngA
  • amino acids 3 to 454 (452 residues), 577.3 bits, E=3.3e-177
• TIGR00231: small GTP-binding protein domain
  • amino acids 4 to 157 (154 residues), 70.5 bits, E=2.2e-23
  • amino acids 198 to 365 (168 residues), 82.9 bits, E=3.4e-27
• PF02421: FeoB_N
  • amino acids 5 to 153 (149 residues), 57.2 bits, E=6.9e-19
  • amino acids 200 to 363 (164 residues), 45 bits, E=3.9e-15
• PF01926: MMR_HSR1
  • amino acids 5 to 119 (115 residues), 98.7 bits, E=1e-31
  • amino acids 201 to 318 (118 residues), 87.3 bits, E=3.5e-28
• PF00009: GTP_EFTU
  • amino acids 200 to 367 (168 residues), 44.1 bits, E=8e-15
• PF14714: KH_dom-like
  • amino acids 374 to 453 (80 residues), 89.5 bits, E=6.1e-29

Best Hits

Swiss-Prot: 100% identical to DER_SHEON: GTPase Der (der) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K03977, GTP-binding protein (inferred from 100% identity to she:Shewmr4_1232)

MetaCyc: 71% identical to 50S ribosomal subunit stability factor (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein EngA" in subsystem Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See Q8EC36 at UniProt or InterPro

Protein Sequence (487 amino acids)

>SO3308 GTP-binding protein EngA (NCBI ptt file) (Shewanella oneidensis MR-1)
MIPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGRAFLSGYEFIVVDTGGI
DGTEEGIETKMAEQSLAAIEEADVVLFMTDARAGLTAADLSIAQHLRSRQKTTFVVANKI
DGIDADSACAEFWSLGLGEVYQMAAAQGRGVTNMIEYALTPYAEAMGIERQGEEEEVDER
QYTEEEAEAEQKRLQDLPIKLAIIGKPNVGKSTLTNRILGEERVVVYDEPGTTRDSIYIP
MERDGREYVIIDTAGVRRRSKVHEVIEKFSVIKTLKAVEDANVVLLIIDAREGVAEQDLG
LLGFALNAGRALVIAVNKWDGIDQGIKDRVKSELDRRLGFIDFARIHFISALHGTGVGHL
FESIEEAYDSATRRVSTSMLTRIMQMSQDDHQPPLVNGRRVKLKYAHAGGYNPPIVVIHG
NQVSKLPDSYKRYMMNYFRRSLKVVGTPIQLRFQEGDNPFENKVEKLTMSQERRRKRALS
HIKDRKK