Protein Info for SO3287 in Shewanella oneidensis MR-1

Name: purL
Annotation: phosphoribosylformylglycinamidine synthase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01735: phosphoribosylformylglycinamidine synthase
  • amino acids 4 to 1290 (1287 residues), 2038.8 bits, E=0
• PF18076: FGAR-AT_N
  • amino acids 35 to 149 (115 residues), 147.3 bits, E=3.5e-47
• PF18072: FGAR-AT_linker
  • amino acids 170 to 219 (50 residues), 79.6 bits, see alignment (E = 3.9e-26)
• PF02769: AIRS_C
  • amino acids 430 to 586 (157 residues), 118.4 bits, E=6.6e-38
  • amino acids 833 to 961 (129 residues), 56.5 bits, E=7.6e-19
• PF13507: GATase_5
  • amino acids 1038 to 1289 (252 residues), 359 bits, E=2.5e-111

Best Hits

Swiss-Prot: 70% identical to PUR4_ALIF1: Phosphoribosylformylglycinamidine synthase (purL) from Aliivibrio fischeri (strain ATCC 700601 / ES114)

MetaCyc: 69% identical to phosphoribosylformylglycinamide synthetase (Escherichia coli K-12 substr. MG1655)
Phosphoribosylformylglycinamidine synthase. [EC: 6.3.5.3]

Predicted SEED Role

"Phosphoribosylformylglycinamidine synthase, synthetase subunit (EC 6.3.5.3) / Phosphoribosylformylglycinamidine synthase, glutamine amidotransferase subunit (EC 6.3.5.3)" in subsystem De Novo Purine Biosynthesis (EC 6.3.5.3)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of 5-aminoimidazole ribonucleotide biosynthesis (6/6 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis I (5/5 steps found)
• 5-aminoimidazole ribonucleotide biosynthesis II (5/5 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.3

Sequence Analysis Tools

See Q8EC57 at UniProt or InterPro

Protein Sequence (1293 amino acids)

>SO3287 phosphoribosylformylglycinamidine synthase (NCBI ptt file) (Shewanella oneidensis MR-1)
MEIIRGAPALSTFRVQKLMEACVSAALPVRQIYAEYVHLADLSELLKPTEREQLEKILTY
GPAIEAHTPQGSLLFVTPRPGTISPWSSKATDIAHNCGLGKVKRLERGIAYYVESDTLTA
EQQRTLQGLLHDRMVEVVLNDFAKADVLFKRTEPAPFKSVNVLAEGRRALEVANVEMGLA
LAEDEIDYLVENFVRLNRNPNDIELMMFAQANSEHCRHKIFNADWTIDGKAQPKSLFKMI
KNTFEVTPDHVLSAYKDNAAVMEGSVAGRFFPDPNGVYSYHTEPMHVLMKVETHNHPTAI
SPYPGAATGSGGEIRDEGATGRGSKPKAGLTGFSVSNLKIPGFVQPWEGNYGKPDRIVSA
LDIMTEGPLGGAAFNNEFGRPALLGYFRTYEQEVSSHNGVEMRGYHKPIMLAGGLGNIRE
EHVQKGEITVGAKLIVLGGPAMNIGLGGGAASSMASGQSSEDLDFASVQRENPEMERRCQ
EVIDRCWQLGDKNPIQFIHDVGAGGLSNAFPELVNDGDRGGIFNLRNVPSDEPGMSPLEI
WCNESQERYVLSVAAEDLPLFTAICERERAPFAVVGEAIQEQHLTLADSHFDNNPIDLPL
EVLLGKAPKMSRNVVSAKAVSPALEQSQIDVKDAVKRVLSLPTVADKTFLITIGDRTVTG
LVNRDQMVGPWQVPVADCAVTAASFDTYAGEAMSLGERTPLALLDFGASARMAVAESIMN
IAGADIGSFKRIKLSANWMSAAGHPGEDAGLYEAVKAVGEELCPELSLTIPVGKDSMSMK
TAWQQDGVNKTVTSPMSLVITAFGVVQDIRNTVTPELRSDKGETSLLLVDLGAGQNRLGG
SCLAQVYGELGDVAPDLDDAALLRGFFETMQKLVANKLVIAYHDRSDGGLFTTLVEMAFA
GNIGLDIDVEDLQGTDLERLFNEELGAVLQVSRDNAAKIAAQFAIAGVPCHVIGTLADDQ
CITIKDGAREIFSDTRVALRTVWSETTYRMQAMRDNPACALEEFKLKQDETDLGLTVNLS
FDPSEDVAAPYILKGAAPKMAILREQGVNSHVEMAAAFDRAGFESRDVHMSDILSGRISL
EEFQGLVACGGFSYGDVLGAGEGWAKSILFNERARNEFSRFFERDSSFALGVCNGCQMLS
NLKEIIPGSEHWPRFVRNRSERFEARFSLVEVQQSPSLFFQGMAGSRMPIAVSHGEGHAE
FASAQALALAEASGTIALRFVNGKGEIATQYPQNPNGSPNGLTGICTTDGRVTLMMPHPE
RVFRTVANSWHPDNWGEDSPWMRMFRNARVNLG