Protein Info for SO3154 in Shewanella oneidensis MR-1

Name: proS
Annotation: prolyl-tRNA synthetase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00409: proline--tRNA ligase
  • amino acids 1 to 566 (566 residues), 838.9 bits, E=1.2e-256
• PF00587: tRNA-synt_2b
  • amino acids 95 to 458 (364 residues), 125.7 bits, E=3.4e-40
• PF04073: tRNA_edit
  • amino acids 257 to 375 (119 residues), 84 bits, E=1.4e-27
• PF03129: HGTP_anticodon
  • amino acids 475 to 567 (93 residues), 81.8 bits, E=5e-27

Best Hits

Swiss-Prot: 100% identical to SYP_SHEON: Proline--tRNA ligase (proS) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01881, prolyl-tRNA synthetase [EC: 6.1.1.15] (inferred from 100% identity to son:SO_3154)

MetaCyc: 72% identical to proline--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]; Proline--tRNA ligase. [EC: 6.1.1.16, 6.1.1.15]; 6.1.1.15 [EC: 6.1.1.16, 6.1.1.15]; 3.1.1.M26 [EC: 6.1.1.16, 6.1.1.15, 3.1.1.M26]

Predicted SEED Role

"Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type" (EC 6.1.1.15)

MetaCyc Pathways

• tRNA charging (21/21 steps found)
• pyoluteorin biosynthesis (1/7 steps found)
• thiocoraline biosynthesis (1/7 steps found)
• ergotamine biosynthesis (4/12 steps found)
• anguibactin biosynthesis (1/8 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• clorobiocin biosynthesis (1/10 steps found)
• pyochelin biosynthesis (1/11 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• pyrrolomycin biosynthesis (1/14 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (1/25 steps found)
• phosphinothricin tripeptide biosynthesis (1/25 steps found)
• colibactin biosynthesis (7/38 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Cysteine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.16

Use Curated BLAST to search for 3.1.1.M26 or 6.1.1.15 or 6.1.1.16

Sequence Analysis Tools

See Q8ECI8 at UniProt or InterPro

Protein Sequence (570 amino acids)

>SO3154 prolyl-tRNA synthetase (NCBI ptt file) (Shewanella oneidensis MR-1)
MRVSKYLLSTQKETPANAEVISHQLMLRAGMIRRNASGLYSYLPSGLRVLRKVEAIVREE
MNKAGAIEILMPMVQPADLWVETGRWDKFGPELLRFKDRHNRDFVLGPTHEEVITDLIRK
EVSSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHLDMDTLNETYEAMYQA
YSNILSRMGLAFRPVLADTGSIGGSMSHEFHVLAQSGEDLIAYSTGSDYAANIEKAESPM
PTETRGAATKELRLVDTPNAKTIAELVEQFGLDITKTVKTLIVKGATEEAPLVALIVRGD
HELNEIKADKLDLVASPLEFAPEALIRDAIGAGPGSLGPVGLNMPIIIDHSVSVMSDFAA
GANVDDKHYFGINWERDLPLAQAADIRNVVEGEPTPDGLGTYAMARGIEVGHIFQLGTNY
SKSMNATVLDENGKSQVLLMGCYGVGVSRIVAAAIEQNFDDRGIVWPEAIAPFSVGILPM
NMHKSHRVTDIAEQLYKDLSAAGIDVLLDDRKERPGVMFADMELIGIPHTVVIGDRNIDA
GVFEYKNRRTGEKQDVPFDQIVDFLKNLQA