Protein Info for SO2834 in Shewanella oneidensis MR-1

Name: nrdD
Annotation: anaerobic ribonucleoside-triphosphate reductase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03477: ATP-cone
  • amino acids 3 to 88 (86 residues), 67.4 bits, E=2e-22
• TIGR02487: anaerobic ribonucleoside-triphosphate reductase
  • amino acids 114 to 704 (591 residues), 659.9 bits, E=1.7e-202
• PF13597: NRDD
  • amino acids 115 to 704 (590 residues), 624.5 bits, E=2.8e-191
• PF01228: Gly_radical
  • amino acids 581 to 685 (105 residues), 103.7 bits, E=1.2e-33

Best Hits

Swiss-Prot: 78% identical to NRDD_SALTY: Anaerobic ribonucleoside-triphosphate reductase (nrdD) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00527, ribonucleoside-triphosphate reductase [EC: 1.17.4.2] (inferred from 80% identity to ddc:Dd586_0360)

MetaCyc: 79% identical to anaerobic ribonucleoside-triphosphate reductase (Escherichia coli K-12 substr. MG1655)
RIBONUCLEOSIDE-TRIP-REDUCT-RXN [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]; 1.1.98.6 [EC: 1.1.98.6]

Predicted SEED Role

"Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2)" in subsystem Ribonucleotide reduction (EC 1.17.4.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (8/8 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis II (7/7 steps found)
• superpathway of adenosine nucleotides de novo biosynthesis II (7/7 steps found)
• adenosine deoxyribonucleotides de novo biosynthesis II (4/4 steps found)
• guanosine deoxyribonucleotides de novo biosynthesis II (4/4 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.98.6 or 1.17.4.2

Sequence Analysis Tools

See Q8EDC8 at UniProt or InterPro

Protein Sequence (705 amino acids)

>SO2834 anaerobic ribonucleoside-triphosphate reductase (NCBI ptt file) (Shewanella oneidensis MR-1)
MPVVIKRDGCRTPFDETRIRDAVIAAANSVGIEDTDYAATVAACVSARVANMAEVEIHHL
QDVVENLLMEGPYKSLARVYIEYRHDRDICRDATSKLNLEIRGLVEQSNAALLNENANKD
SKVIPTQRDLLAGIVAKHYAKSHILPKDVVAAHEMGEIHYHDLDYSPFFPMFNCMLIDLA
GMLTHGFKMGNAEIETPKSISTATAVTAQIIAQVASHIYGGTTINRIDEVLAPFVAKSYD
KHYQVALNWQIKDAKAYATAQTEKECHDAFQSLEYEVNTLHTANGQTPFVTFGFGLGTSW
ESRLIQQSMLKVRMAGLGKNRKTAVFPKLVFAIRDGINHKVGDCNYDVKQLALKCATMRM
YPDILNYEQVEKVTGSFKTPMGCRSFLGSYKENGELVHEGRNNLGVVSLNLPRVALEAKG
DEAEFYRILDERLLLARRALDTRIERLNGVKARVAPILYMEGACGVRLRADDDIAQIFKN
GRASISLGYIGLHETINALYGTETHVFDNAQLREKAVAIVAYLKAATDAWKAETGYGFSL
YSTPSESLCSRFCKLDARQFGVVTGVTDKGYYTNSFHLDVEKRVNPYDKIDFEQPYPAIA
NGGFICYGEYPNMQHNIEALENVWDYSYSRVPYYGTNTPIDECYDCGFTGEFSCTSKGFT
CPKCGNHEPSRVSVTRRVCGYLGSPDARPFNHGKQEEVKRRIKHL