Protein Info for SO2737 in Shewanella oneidensis MR-1

Name: bioD
Annotation: dethiobiotin synthase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13500: AAA_26
  • amino acids 2 to 217 (216 residues), 117.4 bits, E=7.3e-38
• PF01656: CbiA
  • amino acids 3 to 212 (210 residues), 35.2 bits, E=1.1e-12
• TIGR00347: dethiobiotin synthase
  • amino acids 3 to 180 (178 residues), 142.1 bits, E=9.2e-46

Best Hits

Swiss-Prot: 55% identical to BIOD_CELJU: ATP-dependent dethiobiotin synthetase BioD (bioD) from Cellvibrio japonicus (strain Ueda107)

KEGG orthology group: K01935, dethiobiotin synthetase [EC: 6.3.3.3] (inferred from 100% identity to son:SO_2737)

Predicted SEED Role

"Dethiobiotin synthetase (EC 6.3.3.3)" in subsystem Biotin biosynthesis (EC 6.3.3.3)

MetaCyc Pathways

• biotin biosynthesis I (14/15 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate I (4/4 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate II (3/4 steps found)
• biotin biosynthesis II (4/6 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate III (3/5 steps found)

KEGG Metabolic Maps

• Biotin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.3.3

Sequence Analysis Tools

See Q8EDK9 at UniProt or InterPro

Protein Sequence (231 amino acids)

>SO2737 dethiobiotin synthase (NCBI ptt file) (Shewanella oneidensis MR-1)
MFFVTGTDTDSGKTLVSTALLTAFNRDVLRAGSSRLSLGVKPVASGCEITEKGLRNSDAL
KLMSASSLKLSYEQVNPISFEPAIAPHIAAKQQRVNLSPEAILAKLDRQAWAQADFCLIE
GAGGWRLPLGEGRYLSELVQQLNLPVILVVGMKLGCLNHALLTQEAILADGLTVAGWVAN
CVDPNMSVFDENLASLKEMMVSPFLGCIPHLKQADAEHAANFMNIQPLLMK