Protein Info for SO2492 in Shewanella oneidensis MR-1

Updated annotation (from data): acyl-CoA dehydrogenase (1.3.8.-)
Rationale: Specifically important for: Tween 20. Tween 20 hydrolyzes to a mix of C12, C14, and C16 fatty acids; this is probably part of beta oxidation. Similar to fadE from E. coli. The specific SEED annotation as butyryl-CoA dehydrogenase seems questionable but could still be correct
Original annotation: oxidoreductase, acyl-CoA dehydrogenase family (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 759 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details transmembrane" amino acids 544 to 563 (20 residues), see Phobius details PF02771: Acyl-CoA_dh_N" amino acids 85 to 191 (107 residues), 65.7 bits, see alignment E=9.7e-22 PF02770: Acyl-CoA_dh_M" amino acids 195 to 294 (100 residues), 33.6 bits, see alignment E=7e-12 PF00441: Acyl-CoA_dh_1" amino acids 318 to 450 (133 residues), 44.9 bits, see alignment E=2.8e-15 PF09317: ACDH_C" amino acids 472 to 756 (285 residues), 365.8 bits, see alignment E=3.9e-113

Best Hits

Swiss-Prot: 53% identical to FADE_ECOLI: Acyl-coenzyme A dehydrogenase (fadE) from Escherichia coli (strain K12)

KEGG orthology group: K00257, [EC: 1.3.99.-] (inferred from 100% identity to son:SO_2492)

MetaCyc: 53% identical to acyl-CoA dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN-17775 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; RXN-17796 [EC: 1.3.8.8, 1.3.8.7]; 1.3.8.- [EC: 1.3.8.8, 1.3.8.7]

Predicted SEED Role

"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7, 1.3.99.-, 1.3.99.2

Use Curated BLAST to search for 1.3.8.- or 1.3.8.7 or 1.3.8.8 or 1.3.99.- or 1.3.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EE95 at UniProt or InterPro

Protein Sequence (759 amino acids)

>SO2492 acyl-CoA dehydrogenase (1.3.8.-) (Shewanella oneidensis MR-1)
MLTIIIIALIAIIALFAVKSLRMQFITQPVFHFFKKVLPPLSDTEREAMEAGDVWWEGEL
FRGNPNWNTLHSYGKPTLTAEEKDFIDNQVMTALTMIDDFDIVHNRKDLPPELWDYFKKE
GFFALIIPKKFGGKAFSAYANSTIVSKLASRSVSAAVTVMVPNSLGPGELLTHYGTEEQK
ERWLPALAKGDEIPCFALTGPEAGSDAGAIPDVGIVCRGEFNGEEVLGLKLTWNKRYITL
APVATVLGLAFQMRDPDGLLGEKKNLGITCALIPTDHPGVVIGRRHNPLNMAFMNGTTQG
DEVFIPLDWIIGGPEFAGRGWRMLVECLSAGRGISLPALATASGHMATKTTTAYSYVRHQ
FGMAIGQFEGVQEALARIIANTYQLEAARRLTTTGIDLKVKPSVVTAIAKFHMTELGRAV
MNDAMDIQSGKGIQLGPKNYLGHPYMSNPISITVEGANILTRSLMIFGQGATRCHPYVLA
EMEAAAMENQHDALTRFDSLLMGHMGYATRNAFSALFNALTASRFGNAPVSGETKQYYKD
MSRLSSALAFMTDISMLIMGGDLKRKEMLSARMGDVLSQLYLGSATLKLFEDNGRQQDDL
PAVRYVMANRLHLAAKALEDVIRNFPNRPVAWLLRALIFPIGNHFNAPSDKMATELVSGM
LKPSPARERITFLCPEFEGDVGGIAEVEQAFVAQYACKEIYKKLKKAQRSGELPAKVPNL
VLFAKALEQGTISNDEHQTLLHADKLRLAAINVNDFEAL