Protein Info for SO2471 in Shewanella oneidensis MR-1

Name: dapE
Annotation: succinyl-diaminopimelate desuccinylase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 384 TIGR01246: succinyl-diaminopimelate desuccinylase" amino acids 13 to 382 (370 residues), 562.3 bits, see alignment E=2.4e-173 PF01546: Peptidase_M20" amino acids 71 to 380 (310 residues), 148.9 bits, see alignment E=2e-47 PF07687: M20_dimer" amino acids 184 to 291 (108 residues), 103.2 bits, see alignment E=7.6e-34

Best Hits

Swiss-Prot: 100% identical to DAPE_SHEON: Succinyl-diaminopimelate desuccinylase (dapE) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01439, succinyl-diaminopimelate desuccinylase [EC: 3.5.1.18] (inferred from 100% identity to son:SO_2471)

MetaCyc: 64% identical to succinyl-diaminopimelate desuccinylase (Escherichia coli K-12 substr. MG1655)
Succinyl-diaminopimelate desuccinylase. [EC: 3.5.1.18]

Predicted SEED Role

"N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18)" in subsystem Arginine Biosynthesis extended or Lysine Biosynthesis DAP Pathway (EC 3.5.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.5.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EEB6 at UniProt or InterPro

Protein Sequence (384 amino acids)

>SO2471 succinyl-diaminopimelate desuccinylase (NCBI ptt file) (Shewanella oneidensis MR-1)
MTDAAHSSQTGPVTELTKALIARPSVTPLDEGCQTLMAERLAAIGFNIEAMVFEDTTNMW
ARRGNTGPVFCFAGHTDVVPTGDLSRWHTPPFEPTIIDGYLYGRGAADMKGSLAAMVVAT
ERFVAKHPNHPGSIAFLITSDEEGPFINGTTRVIDTLEARNEKITWALVGEPSSTLKLGD
VVKNGRRGSLTANLTVKGVQGHVAYPHLADNPIHKAAPFLAELSQTHWDNGNAFFPPTSM
QIANINGGTGASNVIPGALEVMFNFRYSTEVTAEILIERVETLLTAHELDYDIRWTFNGL
PFLTGEGPLLDATRHAIRQVTGYDTDPQTTGGTSDGRFIAPTGAKVLELGPVNATIHKVN
ECVKVDDLEQLALCYEVILEQLLC