Protein Info for SO2443 in Shewanella oneidensis MR-1

Name: thiF
Annotation: thiF protein, putative (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 transmembrane" amino acids 214 to 230 (17 residues), see Phobius details PF00899: ThiF" amino acids 19 to 278 (260 residues), 242.3 bits, see alignment E=2.1e-76

Best Hits

KEGG orthology group: K03148, adenylyltransferase [EC: 2.7.7.-] (inferred from 100% identity to son:SO_2443)

Predicted SEED Role

"Sulfur carrier protein adenylyltransferase ThiF" in subsystem Thiamin biosynthesis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.-

Use Curated BLAST to search for 2.7.7.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EED9 at UniProt or InterPro

Protein Sequence (300 amino acids)

>SO2443 thiF protein, putative (NCBI ptt file) (Shewanella oneidensis MR-1)
MSATAQKSSSLNDKDFIRYSRQIFLPEVGEAGLLQLKQCHVVIIGCGGLGQLAAQYLACA
GIGSITLVDDDRVELSNLPRQLLFNEADIGQYKAWVAKQKLAVLAPDCQLFAFVQMLTLD
SAESLLAHVATANRQQSALLLDCTDNLAARHCINQLCIEHALPLVSASIAAFNGQLFAVD
QQRFPAGGCYHCVFPADTYAPQNCSNQGVLGPSVGVMASMQALLAIQLLLTATVPNLEHS
TKPQAVGAEHLLGRFWRFDAKSLQWHSARLTRDPECEVCAPSWNTNTDHVLKSVHKGELL