Protein Info for SO2436 in Shewanella oneidensis MR-1

Annotation: methyltransferase, putative (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00452: tRNA (mo5U34)-methyltransferase
  • amino acids 7 to 320 (314 residues), 499 bits, E=2.1e-154
• PF08003: Methyltransf_9
  • amino acids 7 to 322 (316 residues), 562.3 bits, E=7.7e-173
• PF13489: Methyltransf_23
  • amino acids 121 to 266 (146 residues), 41.7 bits, E=3.2e-14
• PF13847: Methyltransf_31
  • amino acids 123 to 228 (106 residues), 34.7 bits, E=4.2e-12
• PF13649: Methyltransf_25
  • amino acids 126 to 220 (95 residues), 42.4 bits, E=2.8e-14
• PF08241: Methyltransf_11
  • amino acids 127 to 224 (98 residues), 40.5 bits, E=1.1e-13
• PF08242: Methyltransf_12
  • amino acids 127 to 222 (96 residues), 39.8 bits, E=1.9e-13

Best Hits

Swiss-Prot: 100% identical to CMOB_SHEON: tRNA U34 carboxymethyltransferase (cmoB) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K15257, tRNA (mo5U34)-methyltransferase [EC: 2.1.1.-] (inferred from 100% identity to son:SO_2436)

MetaCyc: 64% identical to tRNA U34 carboxymethyltransferase (Escherichia coli K-12 substr. MG1655)
RXN0-7067

Predicted SEED Role

"tRNA (5-methoxyuridine) 34 synthase"

MetaCyc Pathways

• 5-(methoxycarbonylmethoxy)uridine biosynthesis (3/3 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis I
• Anthocyanin biosynthesis
• Benzoxazinone biosynthesis
• Biosynthesis of alkaloids derived from shikimate pathway
• Carotenoid biosynthesis - General
• Flavonoid biosynthesis
• Histidine metabolism
• Insect hormone biosynthesis
• Naphthalene and anthracene degradation
• Phenylpropanoid biosynthesis
• Porphyrin and chlorophyll metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• Ubiquinone and menaquinone biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.-

Use Curated BLAST to search for 2.1.1.-

Sequence Analysis Tools

See Q8EEE6 at UniProt or InterPro

Protein Sequence (330 amino acids)

>SO2436 methyltransferase, putative (NCBI ptt file) (Shewanella oneidensis MR-1)
MINFSSFYQQIADSNLQHWLETLPAILGKWQREHKHGNLPKWEKVLNKLHYPSPDSVDLV
DSVTIGTGEQLTPGEKEKLENLLRLFMPWRKGPFHIHGIHIDTEWRSDWKWDRVKPHISP
LQNRTVLDVGCGSGYHMWRMLGAGAKRVVGIDPSPLFLCQFEAVKRLSGENHPVHLLPLG
IEELPPLDAFDTVFSMGVLYHRRSPIDHLLQLRDQLRMGGELVLETLVIDGDENAVLVPQ
DRYGKMNNVWFIPSVAALMLWLKKCDFTDIRCVDTDVTALAEQRRTDWMPNESLVEYLDP
KDITKTVEGYPAPKRATIIAVKNQPNQDLS