Protein Info for SO2433 in Shewanella oneidensis MR-1

Name: aspS
Annotation: aspartyl-tRNA synthetase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 591 TIGR00459: aspartate--tRNA ligase" amino acids 1 to 585 (585 residues), 836.7 bits, see alignment E=4.4e-256 PF01336: tRNA_anti-codon" amino acids 18 to 95 (78 residues), 57.9 bits, see alignment E=1.2e-19 PF00152: tRNA-synt_2" amino acids 119 to 557 (439 residues), 412.8 bits, see alignment E=1.2e-127 PF02938: GAD" amino acids 309 to 408 (100 residues), 97.7 bits, see alignment E=5.6e-32

Best Hits

Swiss-Prot: 100% identical to SYD_SHEON: Aspartate--tRNA ligase (aspS) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01876, aspartyl-tRNA synthetase [EC: 6.1.1.12] (inferred from 100% identity to son:SO_2433)

MetaCyc: 69% identical to aspartate--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Aspartate--tRNA ligase. [EC: 6.1.1.12, 6.1.1.23]; 6.1.1.12 [EC: 6.1.1.12, 6.1.1.23]

Predicted SEED Role

"Aspartyl-tRNA synthetase (EC 6.1.1.12)" in subsystem tRNA aminoacylation, Asp and Asn (EC 6.1.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.12 or 6.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EEE9 at UniProt or InterPro

Protein Sequence (591 amino acids)

>SO2433 aspartyl-tRNA synthetase (NCBI ptt file) (Shewanella oneidensis MR-1)
MRSHYCGDVNKSHVGQEVTLVGWVNRSRDLGGVVFLDLRDREGLVQVVYDPDLPEVFNVA
STLRAEFCVQVKGVVRARPDSQVNAQMKTGEIEVLGKALTIINSSEPLPLSLDNYQNNSE
EQRLKYRYLDLRRPEMAQRLMFRAKVTSAVRRFLDSNGFLDIETPILTKATPEGARDYLV
PSRTYKGQFFALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDIETSF
MTSEQVMNKTEEMMRGLFLEMLNVDLGEFPRMTYNEAMRRFGSDKPDLRNPLELVDVADL
LKDVEFAVFSGPANDEEGRVAALRIPGGASLSRKQIDDYTKFVGIYGAKGLAWMKLNDLT
QGLEGIQSPVLKFLNENIVNEIINRTGAQTGDIILFGADQATVVAESMGALRLKAGEDFN
LLEGQWRPLWVVDFPMFEKINGSFHAVHHPFTAPRGVTPQELEANPANRVSDAYDMVLNG
CELGGGSVRIHNQEMQSAVFRILGITDEEAKEKFGFLLEALRYGTPPHAGLAFGLDRIIM
LMTGASSIRDVMAFPKTTTAACPLTNAPGFANPQQLAELGIAVVKTAKTED