Protein Info for SO2430 in Shewanella oneidensis MR-1

Name: ruvA
Annotation: Holliday junction DNA helicase RuvA (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 205 PF01330: RuvA_N" amino acids 1 to 62 (62 residues), 78.6 bits, see alignment E=6.1e-26 TIGR00084: Holliday junction DNA helicase RuvA" amino acids 1 to 205 (205 residues), 199.2 bits, see alignment E=2.4e-63 PF14520: HHH_5" amino acids 72 to 130 (59 residues), 48.3 bits, see alignment E=2.3e-16 PF07499: RuvA_C" amino acids 161 to 204 (44 residues), 55.9 bits, see alignment 1e-18

Best Hits

Swiss-Prot: 100% identical to RUVA_SHEON: Holliday junction ATP-dependent DNA helicase RuvA (ruvA) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K03550, holliday junction DNA helicase RuvA (inferred from 98% identity to she:Shewmr4_1901)

Predicted SEED Role

"Holliday junction DNA helicase RuvA" in subsystem DNA-replication or RuvABC plus a hypothetical

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EEF2 at UniProt or InterPro

Protein Sequence (205 amino acids)

>SO2430 Holliday junction DNA helicase RuvA (NCBI ptt file) (Shewanella oneidensis MR-1)
MIGRLRGVLIEKQAPEVLIDVNGVGYELQMPLTSFYELPELNQPTTVYTHFVVREDAQLL
YGFITKQERSLFRLLIKANGVGPKLALTILSGMTASEFVGCVERDDIVTLVKLPGVGKKT
AERLLVEMRDKLKSLMEASVGSEREFVLQSNYSPAPVVNSAEEDAISALISLGYKPPQAS
KAVSAAFKEGMDSETLIKAALKSML