Protein Info for SO2411 in Shewanella oneidensis MR-1

Name: gyrA
Annotation: DNA gyrase, A subunit (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 919 TIGR01063: DNA gyrase, A subunit" amino acids 8 to 863 (856 residues), 1220 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 32 to 506 (475 residues), 522.1 bits, see alignment E=1.2e-160 PF03989: DNA_gyraseA_C" amino acids 537 to 584 (48 residues), 38.3 bits, see alignment 7.1e-14 amino acids 587 to 636 (50 residues), 38.1 bits, see alignment 8.6e-14 amino acids 644 to 688 (45 residues), 39.6 bits, see alignment 2.9e-14 amino acids 691 to 708 (18 residues), 11.4 bits, see alignment (E = 1.8e-05) amino acids 734 to 761 (28 residues), 27.6 bits, see alignment (E = 1.5e-10) amino acids 768 to 812 (45 residues), 45.9 bits, see alignment 3e-16 amino acids 817 to 863 (47 residues), 47 bits, see alignment 1.4e-16

Best Hits

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EEH1 at UniProt or InterPro

Protein Sequence (919 amino acids)

>SO2411 DNA gyrase, A subunit (NCBI ptt file) (Shewanella oneidensis MR-1)
MTDLASSISPINIEDELKNSYLDYAMSVIVGRALPDVRDGLKPVHRRVLFAMSELKNDWN
KPYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYTLVDGQGNFGSVDGDSAAAM
RYTEIRMDKLAHQLLADLEKETVDYVPNYDGTEQIPAVLPTRVPNLLINGSSGIAVGMAT
NIPPHNLTEVVKGCLALIADPALSIEQLMDYIPGPDFPTAAIINGRKGIIDAYKTGRGRA
IMRALAEVETEENGRERIIVTEIPYQVNKARLIEKIAELVKDKKLEGISGLRDESDKDGM
RIVIEIKRGEVGEVVLNNLYAQTQMQCSFGINMVALTNGQPKLFNLKEMLECFILHRREV
VTRRTVFELRKARERAHILEALAVALANIDPIIALIKASPTPADAKVKLVEQGWALGHVQ
GMLEKAGDDAARPEWLEPEYGIRDGLYYLTEQQAQAILELRLHRLTGLEHDKIIAEYEEL
LEFIAGLLFILRSPERLMEVIKEELEEILAEYGDARRTVINANEIDMSLEDLINEEDVVV
TLSHLGYAKYQPLSDYQAQRRGGKGKVATKVKDEDFVEKLLVANTHDTILCFSDFGKMYW
LKVYQLPLASRTSRGRPIVNLLPLADGERITAILPVREYAEDKYIIMATSNGTVKKTALT
AYSNPRANGIIAVNLKDGDQLIGVDITNGDDEIMLFSNEGKVVRFKEGEEVAVLDENGNP
VLDEEGNPQINFKGVRPMGRGATGVRGIKLEEGQRVVSLIVPKDDGAILTVTENGYGKRT
ELSEYPSKSRATKGVVSIKVSERNGAVVGAVQVGGNDEIMLISDKGTLVRTPANGVSIIG
RNTQGVTIIRTASDEKVVGLQRIDEIQGDEDEVELDENGMPIAPVIAEGDSVQEEPLEEE
DELEELDEDEELDDEQDED