Protein Info for SO2404 in Shewanella oneidensis MR-1

Name: aroA
Annotation: 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 426 PF00275: EPSP_synthase" amino acids 8 to 418 (411 residues), 480.2 bits, see alignment E=2.6e-148 TIGR01356: 3-phosphoshikimate 1-carboxyvinyltransferase" amino acids 14 to 424 (411 residues), 463.1 bits, see alignment E=4.1e-143

Best Hits

Swiss-Prot: 100% identical to AROA_SHEON: 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K00800, 3-phosphoshikimate 1-carboxyvinyltransferase [EC: 2.5.1.19] (inferred from 100% identity to son:SO_2404)

MetaCyc: 68% identical to 3-phosphoshikimate 1-carboxyvinyltransferase (Escherichia coli K-12 substr. MG1655)
3-phosphoshikimate 1-carboxyvinyltransferase. [EC: 2.5.1.19]

Predicted SEED Role

"5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) (EC 2.5.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EEH8 at UniProt or InterPro

Protein Sequence (426 amino acids)

>SO2404 3-phosphoshikimate 1-carboxyvinyltransferase (NCBI ptt file) (Shewanella oneidensis MR-1)
MKQLRLEPVVQVRGEINIPGSKSISNRALLLATLAQGTTTLTNLLDSDDIRHMLASLKQL
GVEYRLSHNNTVCELAGLGGVMSSKQAQTLFLGNAGTAMRPLCAALTLGQGEFTLTGEPR
MEERPIGDLVDALRQLGANIVYLKNDGFPPLTINATGLNGGDVEIVGDLSSQFLTALLMV
APLAKGSVNIHVKGELVSKPYIDITLALMAQFGVKVINHHYARFEIPAGQHYVSPGKVLV
EGDASSASYFLAAGAIKGGEVKVTGVGRLSIQGDVKFADVLEKMGADIEWGDDYIIARGA
PLTAVDLDMNHIPDAAMTIATAALFAKGTTVIRNIYNWRIKETDRLAAMATELRKVGAEV
EEGNDYIKITPPAVLNTAQIDTYNDHRMAMCFSMLAFADCGITINDPDCTSKTFPDYFVQ
FASLKV