Protein Info for SO2264 in Shewanella oneidensis MR-1

Name: iscS
Annotation: cysteine desulfurase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02006: cysteine desulfurase IscS
  • amino acids 1 to 404 (404 residues), 768.3 bits, E=8.8e-236
• PF00266: Aminotran_5
  • amino acids 5 to 367 (363 residues), 302.2 bits, E=7.8e-94
• PF01212: Beta_elim_lyase
  • amino acids 51 to 234 (184 residues), 35 bits, E=1.5e-12
• PF01041: DegT_DnrJ_EryC1
  • amino acids 66 to 186 (121 residues), 24.2 bits, E=2.8e-09

Best Hits

Swiss-Prot: 100% identical to ISCS_SHEON: Cysteine desulfurase IscS (iscS) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K04487, cysteine desulfurase [EC: 2.8.1.7] (inferred from 100% identity to son:SO_2264)

MetaCyc: 80% identical to cysteine desulfurase IscS (Escherichia coli K-12 substr. MG1655)
Cysteine desulfurase. [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]

Predicted SEED Role

"Cysteine desulfurase (EC 2.8.1.7), IscS subfamily" in subsystem Alanine biosynthesis or Thiamin biosynthesis (EC 2.8.1.7)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis I (10/10 steps found)
• thiazole component of thiamine diphosphate biosynthesis I (6/6 steps found)
• superpathway of L-alanine biosynthesis (4/4 steps found)
• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• molybdopterin biosynthesis (5/6 steps found)
• L-alanine biosynthesis III (1/1 steps found)
• L-cysteine degradation IV (1/1 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (5/7 steps found)
• cytidylyl molybdenum cofactor sulfurylation (1/2 steps found)
• bis(guanylyl molybdopterin) cofactor sulfurylation (1/3 steps found)
• tRNA-uridine 2-thiolation (mammalian mitochondria) (1/4 steps found)
• tRNA-uridine 2-thiolation (yeast mitochondria) (1/4 steps found)
• tRNA-uridine 2-thiolation and selenation (bacteria) (6/11 steps found)
• tRNA-uridine 2-thiolation (thermophilic bacteria) (1/5 steps found)
• [2Fe-2S] iron-sulfur cluster biosynthesis (3/10 steps found)
• tRNA-uridine 2-thiolation (cytoplasmic) (1/8 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.7

Use Curated BLAST to search for 2.8.1.7

Sequence Analysis Tools

See Q8EEU9 at UniProt or InterPro

Protein Sequence (404 amino acids)

>SO2264 cysteine desulfurase (NCBI ptt file) (Shewanella oneidensis MR-1)
MKLPIYLDYAATTPVDPRVAEKMFQYMTMDGIFGNPASRSHRYGWQAEEAVDVARNQVAD
LINADHREIVFTSGATESNNLAIKGVAHFYKKKGKHIITSKTEHKAVLDTCRQLEREGFE
VTYLEPESNGIIPMARLEAAMRDDTILVSIMHVNNEIGVIHDIDAIGELCRSKGIIFHMD
AAQSAGKLPIDVQATKVDLISISGHKMYGPKGIGALYVRRKPRIRLEAQMHGGGHERGMR
SGTLPTHQIVGLGEASAIAKAEMATDNVRIAKLRDKLWNGIKHIEETYVNGDMNQRTSGS
LNVSFNYVEGESLMMALKDLAVSSGSACTSASLEPSYVLRALGLNDEMAHSSIRFSIGRF
TTEEEIDHAIEVITQSIDKLREMSPLWEMFKDGIDLNQVQWAHH