Protein Info for SO2086 in Shewanella oneidensis MR-1

Name: pheT
Annotation: phenylalanyl-tRNA synthetase, beta subunit (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 795 TIGR00472: phenylalanine--tRNA ligase, beta subunit" amino acids 1 to 794 (794 residues), 910.4 bits, see alignment E=5.4e-278 PF01588: tRNA_bind" amino acids 45 to 145 (101 residues), 80.9 bits, see alignment E=1.5e-26 PF03483: B3_4" amino acids 212 to 385 (174 residues), 242.1 bits, see alignment E=7e-76 PF03484: B5" amino acids 407 to 471 (65 residues), 70.1 bits, see alignment E=3.7e-23 PF17759: tRNA_synthFbeta" amino acids 474 to 686 (213 residues), 204.4 bits, see alignment E=4.1e-64 PF03147: FDX-ACB" amino acids 701 to 794 (94 residues), 104.4 bits, see alignment E=8.9e-34

Best Hits

Swiss-Prot: 100% identical to SYFB_SHEON: Phenylalanine--tRNA ligase beta subunit (pheT) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01890, phenylalanyl-tRNA synthetase beta chain [EC: 6.1.1.20] (inferred from 100% identity to son:SO_2086)

MetaCyc: 63% identical to phenylalanine--tRNA ligase subunit beta (Escherichia coli K-12 substr. MG1655)
Phenylalanine--tRNA ligase. [EC: 6.1.1.20]; 6.1.1.20 [EC: 6.1.1.20]; 3.1.1.- [EC: 6.1.1.20]

Predicted SEED Role

"Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20)" (EC 6.1.1.20)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.20

Use Curated BLAST to search for 6.1.1.20

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EF99 at UniProt or InterPro

Protein Sequence (795 amino acids)

>SO2086 phenylalanyl-tRNA synthetase, beta subunit (NCBI ptt file) (Shewanella oneidensis MR-1)
MKFSESWLREWVNPAVSREALSHQITMAGLEVDGVDAVAAEFNGVVIGEVVECGLHPDAD
KLRVTKVSVGSSELIDIVCGAPNCRQGLKVAVAMVGAVLPGDFKIKKAKLRGMPSEGMLC
SYSELGIDIDSDGIIELPLDAPLGTDLREYLKLDDAVIEVDLTANRADCLGMVGLAREVG
VLNRQAVTEPQWQAVTPTTDAKVTINVKESAACPRYLGRVVKNVNVKAATPLWMQEKLRR
SGIRSIDPIVDITNFVLVEFGQPMHAFDLAKLTGDIQVRLGNGEEKITLLDGSEVTIPSD
TLVIADDARPLALAGVFGGEYSGVSDTTQDILLECAFFAPLAIMGKSRRLGLHTDSSHRF
ERGVDPEMQHKVIDRATRLVLDICGGEAGPVVEAKSDADLPKPAQILLRRSKLDKILGHY
VPDSDVVEILERLGFSVVKGEGCWQVITATYRFDMAIEEDLIEEVARIYGYNNIPNVAPI
ASLRMSDHKETDLSLKRVRSLLVARGFQEAVTYSFVDPKLQNLVHPGEQAMVLPNPISSE
MSAMRLSMFTGLLTAVGYNQSRQQGRVRLFETGLRFVPDINAESGVRQQAMLGCVITGPQ
NDEHWAMESKTVDFFDLKGDLEAIIGLTVSASEFSFRVATHSALHPGQCAEILRNDRVIG
HIGAIHPSLEKPFGLNGKTIVFELELDALLHTSLPLAQAVSKFPANRRDIAVVVDESVSA
GDVMKLIRKVGENQLVGINLFDVYLGKGVEPGKKSLAIALTLQDTTRTLEEKEIAETVES
VVSALKTEFNASLRD