Protein Info for SO2071 in Shewanella oneidensis MR-1

Name: hisB
Annotation: imidazoleglycerol-phosphate dehydratase/histidinol-phosphatase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 363 TIGR01261: histidinol-phosphatase" amino acids 11 to 170 (160 residues), 240.7 bits, see alignment E=7.6e-76 TIGR01662: HAD hydrolase, family IIIA" amino acids 12 to 158 (147 residues), 94.2 bits, see alignment E=1.2e-30 TIGR01656: histidinol-phosphate phosphatase domain" amino acids 12 to 153 (142 residues), 149.5 bits, see alignment E=9.6e-48 PF08645: PNK3P" amino acids 12 to 125 (114 residues), 25.9 bits, see alignment E=1.1e-09 PF13242: Hydrolase_like" amino acids 111 to 160 (50 residues), 25.6 bits, see alignment 1.4e-09 PF00475: IGPD" amino acids 204 to 345 (142 residues), 205.8 bits, see alignment E=4.6e-65

Best Hits

Swiss-Prot: 100% identical to HIS7_SHEON: Histidine biosynthesis bifunctional protein HisB (hisB) from Shewanella oneidensis (strain MR-1)

KEGG orthology group: K01089, imidazoleglycerol-phosphate dehydratase / histidinol-phosphatase [EC: 3.1.3.15 4.2.1.19] (inferred from 100% identity to son:SO_2071)

MetaCyc: 61% identical to imidazoleglycerol-phosphate dehydratase / histidinol-phosphatase (Escherichia coli K-12 substr. MG1655)
Imidazoleglycerol-phosphate dehydratase. [EC: 4.2.1.19]; Histidinol-phosphatase. [EC: 4.2.1.19, 3.1.3.15]

Predicted SEED Role

"Histidinol-phosphatase (EC 3.1.3.15) / Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19)" in subsystem Histidine Biosynthesis (EC 3.1.3.15, EC 4.2.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.15 or 4.2.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EFB3 at UniProt or InterPro

Protein Sequence (363 amino acids)

>SO2071 imidazoleglycerol-phosphate dehydratase/histidinol-phosphatase (NCBI ptt file) (Shewanella oneidensis MR-1)
MNPVFTPNVAQKILFIDRDGTLIEEPVTDKQVDNLAKLVFEPQVIPALLRLQKAGFRLVM
VSNQDGLGTPSFPQEDFDAPHNMMMQILSSQGVKFEDVLICPHFNDENCSCRKPKLGLVK
DFLTQGFIDFTQSAVIGDRHTDVELGNAMGIISFQYQRGSLGWNAIADALLNKGRSATVV
RTTKETDIRVTVDLDNASKGTINTGIGFFDHMLDQIATHGNFKMEVNVDGDLEIDDHHSV
EDTALAIGDALRQALGDKRGIARFGFSLPMDEAKGECLLDLSGRPFIKFAAQFEREKVGE
MATEMVPHFFRSFADGLRCTLHVAAEGDNDHHKVEALFKVLGRALRQAVKVEGDVLPSSK
GVL