Protein Info for SO2052 in Shewanella oneidensis MR-1

Annotation: hypothetical oxidoreductase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01266: DAO
  • amino acids 30 to 384 (355 residues), 224 bits, E=6.5e-70
• PF00890: FAD_binding_2
  • amino acids 30 to 224 (195 residues), 21.9 bits, E=1.5e-08
• PF13450: NAD_binding_8
  • amino acids 33 to 61 (29 residues), 23.1 bits, see alignment (E = 1.1e-08)

Best Hits

KEGG orthology group: None (inferred from 100% identity to son:SO_2052)

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49)" in subsystem Methionine Biosynthesis (EC 2.5.1.49)

MetaCyc Pathways

• superpathway of L-methionine biosynthesis (by sulfhydrylation) (11/12 steps found)
• L-methionine biosynthesis III (3/4 steps found)
• L-homocysteine biosynthesis (1/2 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (6/10 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (2/6 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (1/5 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (1/5 steps found)

KEGG Metabolic Maps

• Cysteine metabolism
• Methionine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.49

Use Curated BLAST to search for 2.5.1.49

Sequence Analysis Tools

See Q8EFC9 at UniProt or InterPro

Protein Sequence (429 amino acids)

>SO2052 hypothetical oxidoreductase (NCBI ptt file) (Shewanella oneidensis MR-1)
MSAKRCASYYNATIKQESDYPELEGDIHVDVTIIGGGFTGVATAVELSEKGYKVALLEAN
KIAWGATGRNGGQVTGSLSGDAAMTKQLRRHLGQDAEDYVWNLRWRGHDIIKNRVAKYGI
DCDLKFGHIQTAYHPGHMQELQQMFDEANRRGMGEFMTLVPAQEMSAYLASPLYHGGLVN
RRNMHLHSVNLCLGEARAAASLGAQIFEHSQVLDIQQAELATVVTPKGTVKANSVLIAGN
AYHKLGRPKLRGMLFPASLGNCATAKLPEELAKQLNPQNLAVYDCRFVLDYYRLTADNRL
MFGGGTNYSGRDPKNVAAELRPAIERTFPQLKGVDIEFAWAGMAGIVINRIPQLGKISPN
IFYCQGYSGHGVATSHIMAEIMGQAIDGQMQEFDLFAAMRHIRIPLNEWFGNQALALGML
YYTLRENWR