Protein Info for SO1962 in Shewanella oneidensis MR-1

Updated annotation (from data): 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27)
Rationale: Specifically important for utilizing L-Phenylalanine. Automated validation from mutant phenotype: the predicted function (RXN-10815) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: 4-hydroxyphenylpyruvate dioxygenase (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 86% identical to MELA_SHECO: Protein MelA (melA) from Shewanella colwelliana

KEGG orthology group: K00457, 4-hydroxyphenylpyruvate dioxygenase [EC: 1.13.11.27] (inferred from 100% identity to son:SO_1962)

Predicted SEED Role

"4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27)" in subsystem Aromatic amino acid degradation or Homogentisate pathway of aromatic compound degradation or Plastoquinone Biosynthesis or Tocopherol Biosynthesis (EC 1.13.11.27)

MetaCyc Pathways

L-tyrosine degradation I (5/5 steps found)
plastoquinol-9 biosynthesis I (1/3 steps found)
superpathway of plastoquinol biosynthesis (2/5 steps found)
L-phenylalanine degradation IV (mammalian, via side chain) (3/9 steps found)
vitamin E biosynthesis (tocopherols) (1/7 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.13.11.27

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EFK9 at UniProt or InterPro

Protein Sequence (346 amino acids)

>SO1962 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27) (Shewanella oneidensis MR-1)
MASELNPLGLLGIEFTEFASSDTDFMHKVFIDFGFSLLKKAKNKDILYYKQNDINFLLNK
QREGFSAKFAKSHGPAICSMGWRVEDASFAHRVAVERGAKAADDSAKDLPYPAIYGIGDS
LIYFIDTFGANNNIYATDFEDLSEPVITQEKGFIEVDHLTNNVYKGTMEHWANFYKNIFG
FTEVRYFDISGVQTALVSYALRSPDGSFCIPINEGKGNDKNQIDEYLKEYNGPGVQHLAF
RSRDIVKSLDAMEGSSIQCLDIIPEYYDTIFDKVPQVTENRDRIKHHQILVDGDESGYLL
QIFTKNLFGPIFIEIIQRKNNLGFGEGNFTALFQSIERDQMRRGVL