Protein Info for SO1894 in Shewanella oneidensis MR-1

Annotation: acetyl-CoA carboxylase, biotin carboxylase, putative (NCBI ptt file)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 694 PF00289: Biotin_carb_N" amino acids 11 to 120 (110 residues), 152.3 bits, see alignment E=3.1e-48 PF02786: CPSase_L_D2" amino acids 126 to 332 (207 residues), 257.3 bits, see alignment E=5.2e-80 PF02222: ATP-grasp" amino acids 144 to 302 (159 residues), 30.6 bits, see alignment E=1.3e-10 PF07478: Dala_Dala_lig_C" amino acids 150 to 301 (152 residues), 36.3 bits, see alignment E=2.1e-12 PF02785: Biotin_carb_C" amino acids 346 to 453 (108 residues), 127.4 bits, see alignment E=1.2e-40 PF21139: BT_MCC_alpha" amino acids 468 to 605 (138 residues), 33.7 bits, see alignment E=2.1e-11 PF00364: Biotin_lipoyl" amino acids 619 to 683 (65 residues), 57.3 bits, see alignment E=5.5e-19

Best Hits

KEGG orthology group: K01968, 3-methylcrotonyl-CoA carboxylase alpha subunit [EC: 6.4.1.4] (inferred from 100% identity to son:SO_1894)

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4

Use Curated BLAST to search for 6.4.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8EFS2 at UniProt or InterPro

Protein Sequence (694 amino acids)

>SO1894 acetyl-CoA carboxylase, biotin carboxylase, putative (NCBI ptt file) (Shewanella oneidensis MR-1)
MLTKQMLTNSMFTKLLIANRGEIACRIIKTAQAMGVRTVALYSDADKNARHVAMADESFY
LGGSAPADSYLKGDLIIAIAKKAQAQAIHPGYGFLSENADFARKCEAAGIVFVGPGSDAI
DAMGSKSAAKAIMTAAQVPLVPGYHGDDQTDATLKAEALKIGFPMLIKAAYGGGGKGMRI
VEHEGEIMDAINSARREAASSFGNDKLLMERYLRQPRHVEVQVFADTFGNAIYLSDRDCS
IQRRHQKVVEEAPAPGLSDELRAQMGEAAVAAAKAIDYVGAGTIEFLLDTDNSFYFMEMN
TRLQVEHPVTEMVTGQDLVKWQLMVASGQPLPLKQDEVRIHGHAFEVRIYAEDPQNEFLP
ASGKLNFLREPEQSKYVRIDSGIRENDVISNFYDPMIAKLIVWDESRPRALQRLVHALES
YQISGLKHNIEFLANIAEHPAFAKADFSTDFINRYGDALIGSASSEADTALAFAALYQVL
ARKEAAKAQAINSADPDSPWGQVSGFRLNSVSQHSIALLDDAHELQQLVLLDFGDHYQLS
HNRSSQHQLSQSAADGQVSKSLSGELKQDLLLAEINGHKSKVPVSAQGDDFTLFLPSGSY
HFRAVKTQVVEAESSNEDKLKAPMNGTVVTHLVDVGAEVKAGQGLLVMEAMKMEYTIEAP
FDGIVTEFYFKAGELVSDGAVLLHVEPKAQSEEA